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Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation
Gai, Qing-Qing1; Qu, Feng1; Liu, Zong-Jian1; Dai, Rong-Ji1; Zhang, Yu-Kui2; Qu F(屈锋)
关键词Molecularly Imprinted Polymer (Mip) Atom Transfer Radical Polymerization (Atrp) Magnetic Particles Protein Separation
刊名JOURNAL OF CHROMATOGRAPHY A
2010-07-30
DOI10.1016/j.chroma.2010.06.001
1217期:31页:5035-5042
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名2;5
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]GEL COMPOSITE MICROSPHERES ; MAGNETIC-SUSCEPTIBILITY ; RECOGNITION ; HYDROGEL ; BEADS ; NANOPARTICLES ; ADSORPTION ; PARTICLES ; MEMBRANES ; MATRIX
英文摘要Molecular imprinting as a promising and facile separation technique has received much attention because of their high selectivity for target molecules. In this study, the superparamagnetic lysozyme surface-imprinted polymer was prepared by a novel fabricating protocol, the grafting of the imprinted polymer on magnetic particles in aqueous media was done by atom transfer radical polymerization (ATRP), and the properties of the imprinted polymer were characterized in detail. Its high selective adsorption and recognition to lysozyme demonstrated the separation ability of the magnetic imprinted material to template molecule, and it has been used for quick and direct separation of lysozyme from the mixture of standard proteins and real egg white samples under an external magnetic field. Furthermore, the elution of lysozyme from the imprinted material was achieved by PEG/sulphate aqueous two-phase system, which caused lysozyme not only desorption from the imprinted materials but also redistribution in the top and bottom phase of aqueous two-phase system. The aqueous two-phase system exhibited some of the extraction and enrichment effect to desorbed lysozyme. Our results showed that ATRP is a promising method for the protein molecularly imprinted polymer preparation. (C) 2010 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000280231900004
引用统计
被引频次:136[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103209
专题中国科学院大连化学物理研究所
通讯作者Qu F(屈锋)
作者单位1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
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Gai, Qing-Qing,Qu, Feng,Liu, Zong-Jian,et al. Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(31):5035-5042.
APA Gai, Qing-Qing,Qu, Feng,Liu, Zong-Jian,Dai, Rong-Ji,Zhang, Yu-Kui,&屈锋.(2010).Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation.JOURNAL OF CHROMATOGRAPHY A,1217(31),5035-5042.
MLA Gai, Qing-Qing,et al."Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation".JOURNAL OF CHROMATOGRAPHY A 1217.31(2010):5035-5042.
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