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题名: Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation
作者: Gai, Qing-Qing1;  Qu, Feng1;  Liu, Zong-Jian1;  Dai, Rong-Ji1;  Zhang, Yu-Kui2
通讯作者: 屈锋
关键词: Molecularly imprinted polymer (MIP) ;  Atom transfer radical polymerization (ATRP) ;  Magnetic particles ;  Protein separation
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2010-07-30
DOI: 10.1016/j.chroma.2010.06.001
卷: 1217, 期:31, 页:5035-5042
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2;5
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Molecular imprinting as a promising and facile separation technique has received much attention because of their high selectivity for target molecules. In this study, the superparamagnetic lysozyme surface-imprinted polymer was prepared by a novel fabricating protocol, the grafting of the imprinted polymer on magnetic particles in aqueous media was done by atom transfer radical polymerization (ATRP), and the properties of the imprinted polymer were characterized in detail. Its high selective adsorption and recognition to lysozyme demonstrated the separation ability of the magnetic imprinted material to template molecule, and it has been used for quick and direct separation of lysozyme from the mixture of standard proteins and real egg white samples under an external magnetic field. Furthermore, the elution of lysozyme from the imprinted material was achieved by PEG/sulphate aqueous two-phase system, which caused lysozyme not only desorption from the imprinted materials but also redistribution in the top and bottom phase of aqueous two-phase system. The aqueous two-phase system exhibited some of the extraction and enrichment effect to desorbed lysozyme. Our results showed that ATRP is a promising method for the protein molecularly imprinted polymer preparation. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: GEL COMPOSITE MICROSPHERES ;  MAGNETIC-SUSCEPTIBILITY ;  RECOGNITION ;  HYDROGEL ;  BEADS ;  NANOPARTICLES ;  ADSORPTION ;  PARTICLES ;  MEMBRANES ;  MATRIX
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000280231900004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103209
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Gai, Qing-Qing,Qu, Feng,Liu, Zong-Jian,et al. Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(31):5035-5042.
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