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Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation
Gai, Qing-Qing1; Qu, Feng1; Liu, Zong-Jian1; Dai, Rong-Ji1; Zhang, Yu-Kui2; Qu F(屈锋)
KeywordMolecularly Imprinted Polymer (Mip) Atom Transfer Radical Polymerization (Atrp) Magnetic Particles Protein Separation
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2010-07-30
DOI10.1016/j.chroma.2010.06.001
Volume1217Issue:31Pages:5035-5042
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank2;5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordGEL COMPOSITE MICROSPHERES ; MAGNETIC-SUSCEPTIBILITY ; RECOGNITION ; HYDROGEL ; BEADS ; NANOPARTICLES ; ADSORPTION ; PARTICLES ; MEMBRANES ; MATRIX
AbstractMolecular imprinting as a promising and facile separation technique has received much attention because of their high selectivity for target molecules. In this study, the superparamagnetic lysozyme surface-imprinted polymer was prepared by a novel fabricating protocol, the grafting of the imprinted polymer on magnetic particles in aqueous media was done by atom transfer radical polymerization (ATRP), and the properties of the imprinted polymer were characterized in detail. Its high selective adsorption and recognition to lysozyme demonstrated the separation ability of the magnetic imprinted material to template molecule, and it has been used for quick and direct separation of lysozyme from the mixture of standard proteins and real egg white samples under an external magnetic field. Furthermore, the elution of lysozyme from the imprinted material was achieved by PEG/sulphate aqueous two-phase system, which caused lysozyme not only desorption from the imprinted materials but also redistribution in the top and bottom phase of aqueous two-phase system. The aqueous two-phase system exhibited some of the extraction and enrichment effect to desorbed lysozyme. Our results showed that ATRP is a promising method for the protein molecularly imprinted polymer preparation. (C) 2010 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000280231900004
Citation statistics
Cited Times:137[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/103209
Collection中国科学院大连化学物理研究所
Corresponding AuthorQu F(屈锋)
Affiliation1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Gai, Qing-Qing,Qu, Feng,Liu, Zong-Jian,et al. Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(31):5035-5042.
APA Gai, Qing-Qing,Qu, Feng,Liu, Zong-Jian,Dai, Rong-Ji,Zhang, Yu-Kui,&屈锋.(2010).Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation.JOURNAL OF CHROMATOGRAPHY A,1217(31),5035-5042.
MLA Gai, Qing-Qing,et al."Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation".JOURNAL OF CHROMATOGRAPHY A 1217.31(2010):5035-5042.
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