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题名: A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation
作者: Sun, Xiangli1;  Lin, Dong1;  He, Xiwen1;  Chen, Langxing1;  Zhang, Yukui1, 2
通讯作者: 陈朗星 ;  张玉奎
关键词: Click chemistry ;  High performance liquid chromatography ;  In situ ;  Polymer monolithic column ;  Protein separation
刊名: TALANTA
发表日期: 2010-06-30
DOI: 10.1016/j.talanta.2010.03.016
卷: 82, 期:1, 页:404-408
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2;5
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A simple one-step in situ "click" modification strategy was developed for the preparation of hydrophobic organic monolithic columns for the first time. The column morphology and surface chemistry of the fabricated monolithic columns were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, respectively. The chromatographic performances of the C8/C18 "click" monoliths were evaluated through the separation of a mixture of five proteins such as ribonuclease A, soybean trypsin inhibitor, cytochrome c, bovine haemoglobin and bovine serum albumin. Compared with the blank column, the higher hydrophobicity stationary phases obtained from the "clicked" modification have longer retention times and higher resolution for the five proteins. The separation of five proteins mixture on click C18 monolith with gradient elution at different flow rates was also investigated, the baseline separation of five proteins could be achieved at three different flow rates. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ALKYNE 3+2 CYCLOADDITION ;  CONTINUOUS POLYMER BEDS ;  SILICA ;  COLUMNS ;  AZIDES ;  FUNCTIONALIZATION ;  STABILIZATION ;  PEPTIDES ;  LIGATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000279488900058
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103211
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Sun, Xiangli,Lin, Dong,He, Xiwen,et al. A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation[J]. TALANTA,2010,82(1):404-408.
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