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A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation
Sun, Xiangli1; Lin, Dong1; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,2; Chen LX(陈朗星); Zhang YK(张玉奎)
关键词Click Chemistry High Performance Liquid Chromatography In Situ Polymer Monolithic Column Protein Separation
刊名TALANTA
2010-06-30
DOI10.1016/j.talanta.2010.03.016
82期:1页:404-408
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名2;5
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; ALKYNE 3+2 CYCLOADDITION ; CONTINUOUS POLYMER BEDS ; SILICA ; COLUMNS ; AZIDES ; FUNCTIONALIZATION ; STABILIZATION ; PEPTIDES ; LIGATION
英文摘要A simple one-step in situ "click" modification strategy was developed for the preparation of hydrophobic organic monolithic columns for the first time. The column morphology and surface chemistry of the fabricated monolithic columns were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, respectively. The chromatographic performances of the C8/C18 "click" monoliths were evaluated through the separation of a mixture of five proteins such as ribonuclease A, soybean trypsin inhibitor, cytochrome c, bovine haemoglobin and bovine serum albumin. Compared with the blank column, the higher hydrophobicity stationary phases obtained from the "clicked" modification have longer retention times and higher resolution for the five proteins. The separation of five proteins mixture on click C18 monolith with gradient elution at different flow rates was also investigated, the baseline separation of five proteins could be achieved at three different flow rates. (C) 2010 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000279488900058
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103211
专题中国科学院大连化学物理研究所
通讯作者Chen LX(陈朗星); Zhang YK(张玉奎)
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Sun, Xiangli,Lin, Dong,He, Xiwen,et al. A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation[J]. TALANTA,2010,82(1):404-408.
APA Sun, Xiangli.,Lin, Dong.,He, Xiwen.,Chen, Langxing.,Zhang, Yukui.,...&张玉奎.(2010).A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation.TALANTA,82(1),404-408.
MLA Sun, Xiangli,et al."A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation".TALANTA 82.1(2010):404-408.
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