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题名: Selective extraction of low molecular weight proteins by mesoporous silica particles with modified internal and external surfaces
作者: Qi, Yanxia1;  Wu, Dapeng1;  Wei, Junying2;  Ding, Kun1;  Wang, Hua1;  Zhang, Yangjun2;  Qian, Xiaohong2;  Guan, Yafeng1
通讯作者: 关亚风
关键词: Interior and external modification ;  Mesoporous silica ;  Peptides ;  Selective extraction
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2010-10-01
DOI: 10.1007/s00216-010-4081-1
卷: 398, 期:4, 页:1715-1722
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 105
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A new mesoporous silica material with modified external and internal surfaces (alkyl diol-vinyl-SiO(2)) was prepared and applied to selectively extract low molecular weight (LMW) proteins and peptides (less than 11 kDa) from biological samples. X-ray diffraction, N(2) adsorption, transmission electron microscopy, and Fourier transform infrared spectroscopy were used to characterize the alkyl diol-vinyl-SiO(2). This material was used to extract LMW proteins (peptides) from standard proteins, a mixture of peptides, and crude human plasma. We compared the amounts of different proteins adsorbed by alkyl diol-vinyl-SiO(2) and by vinyl-SiO(2) (vinyl group on the internal surface of the mesoporous silica material). The desorption recovery of alkyl diol-vinyl-SiO(2) by different eluents was also investigated by using a standard protein (insulin) as a model LMW protein. The material could efficiently extract LMW proteins and peptides from a mixture of standard proteins and crude human plasma with good extraction efficiency and desorption recovery. Furthermore, the alkyl diol-vinyl-SiO(2) had much better extraction selectivity for LMW proteins and peptides than a commercial C(18) solid phase extraction material.
关键词[WOS]: SOLID-PHASE EXTRACTION ;  LOW-ABUNDANCE PEPTIDES ;  TOF MS ANALYSIS ;  HUMAN PLASMA ;  MCM-41 ;  FUNCTIONALIZATION ;  INTEGRATION ;  ENRICHMENT
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000282090000017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103403
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem CAS, Dalian 116023, Peoples R China
2.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China

Recommended Citation:
Qi, Yanxia,Wu, Dapeng,Wei, Junying,et al. Selective extraction of low molecular weight proteins by mesoporous silica particles with modified internal and external surfaces[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2010,398(4):1715-1722.
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