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题名: Visual detection of sub-femtomole DNA by a gold nanoparticle seeded homogeneous reduction assay: Toward a generalized sensitivity-enhancing strategy
作者: Bai, Xue1;  Shao, Congying1;  Han, Xiaogang1;  Li, Yulin1;  Guan, Yafeng2;  Deng, Zhaoxiang1
通讯作者: 邓兆祥
关键词: DNA assay ;  Gold nanoparticles ;  Sensitivity enhancement ;  Visual detection
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2010-04-15
DOI: 10.1016/j.bios.2010.01.012
卷: 25, 期:8, 页:1984-1988
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 105
产权排名: 2;5
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: Gold nanoparticles (AuNPs) have been employed to design colorimetric visual sensing assays toward the detections of various targets including DNA, based on the aggregation induced color transitions of AuNPs. However, the relatively high detection limit (LOD 10 nM) in the case of DNA detection has become a stumbling block on the road of the further development and applications of these assays. This research aims at overcoming this limit by virtue of a seeded gold reduction strategy. Typically, low concentrations of 13 nm AuNPs modified with suitable DNA probes are allowed to hybridize with a DNA target to form aggregates, which are then transferred into a gold-enhancing cocktail for further depositions of more gold on the initial AuNP seeds. The color of the assay thus sensitively reflects the initial aggregation status of the 13 nm AuNPs, which can be related to the concentrations of the DNA target. This assay has a sensitivity that is at least 25-50 times improved. Under still not fully optimized conditions, 0.4 fmol DNA in a 2 mu L sample can be confidently detected with the ability of distinguishing a single base mutation. It does not require isolations of the 13 nm AuNP aggregates during analyses and shares the advantages of a homogeneous assay, including simplicity, adaptability, convenience, and being free of interferences due to non-specific surface adsorptions. (c) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: WET CHEMICAL SYNTHESIS ;  COLORIMETRIC DETECTION ;  ASPECT-RATIO ;  AU-NANOPARTICLES ;  DNAZYME ;  NANORODS ;  APTAMER ;  DESIGN ;  PROBES ;  IONS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000276424200020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103417
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Sci & Technol China, Dept Chem, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Bai, Xue,Shao, Congying,Han, Xiaogang,et al. Visual detection of sub-femtomole DNA by a gold nanoparticle seeded homogeneous reduction assay: Toward a generalized sensitivity-enhancing strategy[J]. BIOSENSORS & BIOELECTRONICS,2010,25(8):1984-1988.
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