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Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization
Huang, Shuqiang1,2; Li, Chunyu1,2; Lin, Bingcheng1; Qin, Jianhua1; Lin BC(林炳承); Qin JH(秦建华)
刊名LAB ON A CHIP
2010
DOI10.1039/c005227b
10期:21页:2925-2931
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]GENE-EXPRESSION PATTERNS ; MICROARRAY HYBRIDIZATION ; POLY(METHYL METHACRYLATE) ; HETERODUPLEX ANALYSIS ; GEL-ELECTROPHORESIS ; CHIP ; DISCRIMINATION ; IMMOBILIZATION ; POLYMORPHISMS ; DIAGNOSTICS
英文摘要We present a novel microfluidic device integrated with microvalves and micropumps for rapid DNA hybridization using shuttle flow. The device is composed of 48 hybridization units containing 48 microvalves and 96 micropumps for the automation of shuttle flow. We used four serotypes of Dengue Virus genes (18mer) to demonstrate that the automatic shuttle flow shortened the hybridization time to 90 s, reduced sample consumption to 1 mu L and lowered detection limit to 100 pM (100 amol in a 1 mu L sample). Moreover, we applied this device to realize single base discrimination and analyze 48 samples containing different DNA targets, simultaneously. For kinetic measurements of nucleotide hybridization, on-line monitoring of the processes was carried out. This rapid hybridization device has the ability for accommodating the entire hybridization process (i.e., injection, hybridization, washing, detection, signal acquisition) in an automated and high-throughput fashion.
语种英语
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WOS记录号WOS:000282808700015
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被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103437
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承); Qin JH(秦建华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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GB/T 7714
Huang, Shuqiang,Li, Chunyu,Lin, Bingcheng,et al. Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization[J]. LAB ON A CHIP,2010,10(21):2925-2931.
APA Huang, Shuqiang,Li, Chunyu,Lin, Bingcheng,Qin, Jianhua,林炳承,&秦建华.(2010).Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization.LAB ON A CHIP,10(21),2925-2931.
MLA Huang, Shuqiang,et al."Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization".LAB ON A CHIP 10.21(2010):2925-2931.
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