DICP OpenIR
Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization
Huang, Shuqiang1,2; Li, Chunyu1,2; Lin, Bingcheng1; Qin, Jianhua1; Lin BC(林炳承); Qin JH(秦建华)
Source PublicationLAB ON A CHIP
2010
DOI10.1039/c005227b
Volume10Issue:21Pages:2925-2931
Indexed BySCI
SubtypeArticle
Department18
Funding Project1807
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS KeywordGENE-EXPRESSION PATTERNS ; MICROARRAY HYBRIDIZATION ; POLY(METHYL METHACRYLATE) ; HETERODUPLEX ANALYSIS ; GEL-ELECTROPHORESIS ; CHIP ; DISCRIMINATION ; IMMOBILIZATION ; POLYMORPHISMS ; DIAGNOSTICS
AbstractWe present a novel microfluidic device integrated with microvalves and micropumps for rapid DNA hybridization using shuttle flow. The device is composed of 48 hybridization units containing 48 microvalves and 96 micropumps for the automation of shuttle flow. We used four serotypes of Dengue Virus genes (18mer) to demonstrate that the automatic shuttle flow shortened the hybridization time to 90 s, reduced sample consumption to 1 mu L and lowered detection limit to 100 pM (100 amol in a 1 mu L sample). Moreover, we applied this device to realize single base discrimination and analyze 48 samples containing different DNA targets, simultaneously. For kinetic measurements of nucleotide hybridization, on-line monitoring of the processes was carried out. This rapid hybridization device has the ability for accommodating the entire hybridization process (i.e., injection, hybridization, washing, detection, signal acquisition) in an automated and high-throughput fashion.
Language英语
URL查看原文
WOS IDWOS:000282808700015
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/103437
Collection中国科学院大连化学物理研究所
Corresponding AuthorLin BC(林炳承); Qin JH(秦建华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Huang, Shuqiang,Li, Chunyu,Lin, Bingcheng,et al. Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization[J]. LAB ON A CHIP,2010,10(21):2925-2931.
APA Huang, Shuqiang,Li, Chunyu,Lin, Bingcheng,Qin, Jianhua,林炳承,&秦建华.(2010).Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization.LAB ON A CHIP,10(21),2925-2931.
MLA Huang, Shuqiang,et al."Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization".LAB ON A CHIP 10.21(2010):2925-2931.
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