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题名: Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization
作者: Huang, Shuqiang1, 2;  Li, Chunyu1, 2;  Lin, Bingcheng1;  Qin, Jianhua1
通讯作者: 林炳承 ;  秦建华
刊名: LAB ON A CHIP
发表日期: 2010
DOI: 10.1039/c005227b
卷: 10, 期:21, 页:2925-2931
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: We present a novel microfluidic device integrated with microvalves and micropumps for rapid DNA hybridization using shuttle flow. The device is composed of 48 hybridization units containing 48 microvalves and 96 micropumps for the automation of shuttle flow. We used four serotypes of Dengue Virus genes (18mer) to demonstrate that the automatic shuttle flow shortened the hybridization time to 90 s, reduced sample consumption to 1 mu L and lowered detection limit to 100 pM (100 amol in a 1 mu L sample). Moreover, we applied this device to realize single base discrimination and analyze 48 samples containing different DNA targets, simultaneously. For kinetic measurements of nucleotide hybridization, on-line monitoring of the processes was carried out. This rapid hybridization device has the ability for accommodating the entire hybridization process (i.e., injection, hybridization, washing, detection, signal acquisition) in an automated and high-throughput fashion.
关键词[WOS]: GENE-EXPRESSION PATTERNS ;  MICROARRAY HYBRIDIZATION ;  POLY(METHYL METHACRYLATE) ;  HETERODUPLEX ANALYSIS ;  GEL-ELECTROPHORESIS ;  CHIP ;  DISCRIMINATION ;  IMMOBILIZATION ;  POLYMORPHISMS ;  DIAGNOSTICS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000282808700015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103437
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Huang, Shuqiang,Li, Chunyu,Lin, Bingcheng,et al. Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization[J]. LAB ON A CHIP,2010,10(21):2925-2931.
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