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题名: Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans
作者: Shi, Weiwei1;  Wen, Hui1;  Lu, Yao1;  Shi, Yang1;  Lin, Bingcheng1;  Qin, Jianhua1
通讯作者: 林炳承 ;  秦建华
刊名: LAB ON A CHIP
发表日期: 2010
DOI: 10.1039/c0lc00256a
卷: 10, 期:21, 页:2855-2863
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: A droplet-based microfluidic device integrated with a novel floatage-based trap array and a tapered immobilization channel array was presented for characterizing the neurotoxin-induced multiple responses in individual Caenorhabditis elegans (C. elegans) continuously. The established device enabled the evaluations of movement and fluorescence imaging analysis of individual C. elegans simultaneously. The utility of this device was demonstrated by the pharmacological evaluation of neurotoxin (6-hydroxydopamine, 6-OHDA) triggered mobility defects, neuron degeneration and oxidative stress in individual worms. Exposure of living worms to 6-OHDA could cause obvious mobility defects, selective degeneration of dopaminergic (DAergic) neurons, and increased oxidative stress in a dose dependent manner. These results are important towards the understanding of mechanisms leading to DAergic toxicity by neurotoxin and will be of benefit for the screening of new therapeutics for neurodegenerative diseases. This device was simple, stable and easy to operate, with the potential to facilitate whole-animal assays and drug screening in a high throughput manner at single animal resolution.
关键词[WOS]: C-ELEGANS ;  PARKINSONS-DISEASE ;  ON-CHIP ;  OXIDATIVE STRESS ;  ANIMAL-MODELS ;  SYSTEM ;  IMMOBILIZATION ;  DEGENERATION ;  GENETICS ;  NEURONS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000282808700005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103439
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China

Recommended Citation:
Shi, Weiwei,Wen, Hui,Lu, Yao,et al. Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans[J]. LAB ON A CHIP,2010,10(21):2855-2863.
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