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Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans
Shi, Weiwei; Wen, Hui; Lu, Yao; Shi, Yang; Lin, Bingcheng; Qin, Jianhua; Lin BC(林炳承); Qin JH(秦建华)
刊名LAB ON A CHIP
2010
DOI10.1039/c0lc00256a
10期:21页:2855-2863
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]C-ELEGANS ; PARKINSONS-DISEASE ; ON-CHIP ; OXIDATIVE STRESS ; ANIMAL-MODELS ; SYSTEM ; IMMOBILIZATION ; DEGENERATION ; GENETICS ; NEURONS
英文摘要A droplet-based microfluidic device integrated with a novel floatage-based trap array and a tapered immobilization channel array was presented for characterizing the neurotoxin-induced multiple responses in individual Caenorhabditis elegans (C. elegans) continuously. The established device enabled the evaluations of movement and fluorescence imaging analysis of individual C. elegans simultaneously. The utility of this device was demonstrated by the pharmacological evaluation of neurotoxin (6-hydroxydopamine, 6-OHDA) triggered mobility defects, neuron degeneration and oxidative stress in individual worms. Exposure of living worms to 6-OHDA could cause obvious mobility defects, selective degeneration of dopaminergic (DAergic) neurons, and increased oxidative stress in a dose dependent manner. These results are important towards the understanding of mechanisms leading to DAergic toxicity by neurotoxin and will be of benefit for the screening of new therapeutics for neurodegenerative diseases. This device was simple, stable and easy to operate, with the potential to facilitate whole-animal assays and drug screening in a high throughput manner at single animal resolution.
语种英语
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WOS记录号WOS:000282808700005
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被引频次:65[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103439
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承); Qin JH(秦建华)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
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GB/T 7714
Shi, Weiwei,Wen, Hui,Lu, Yao,et al. Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans[J]. LAB ON A CHIP,2010,10(21):2855-2863.
APA Shi, Weiwei.,Wen, Hui.,Lu, Yao.,Shi, Yang.,Lin, Bingcheng.,...&秦建华.(2010).Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans.LAB ON A CHIP,10(21),2855-2863.
MLA Shi, Weiwei,et al."Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans".LAB ON A CHIP 10.21(2010):2855-2863.
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