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题名: Wax-bonding 3D microfluidic chips
作者: Gong, Xiuqing2;  Yi, Xin2;  Xiao, Kang3;  Li, Shunbo2;  Kodzius, Rimantas4;  Qin, Jianhua1;  Wen, Weijia2, 4
通讯作者: Weijia Wen
刊名: LAB ON A CHIP
发表日期: 2010
DOI: 10.1039/c004744a
卷: 10, 期:19, 页:2622-2627
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 4;6
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: We report a simple, low-cost and detachable microfluidic chip incorporating easily accessible paper, glass slides or other polymer films as the chip materials along with adhesive wax as the recycling bonding material. We use a laser to cut through the paper or film to form patterns and then sandwich the paper and film between glass sheets or polymer membranes. The hot-melt adhesive wax can realize bridge bonding between various materials, for example, paper, polymethylmethacrylate (PMMA) film, glass sheets, or metal plate. The bonding process is reversible and the wax is reusable through a melting and cooling process. With this process, a three-dimensional (3D) microfluidic chip is achievable by vacuating and venting the chip in a hot-water bath. To study the biocompatibility and applicability of the wax-based microfluidic chip, we tested the PCR compatibility with the chip materials first. Then we applied the wax-paper based microfluidic chip to HeLa cell electroporation (EP). Subsequently, a prototype of a 5-layer 3D chip was fabricated by multilayer wax bonding. To check the sealing ability and the durability of the chip, green fluorescence protein (GFP) recombinant Escherichia coli (E. coli) bacteria were cultured, with which the chemotaxis of E. coli was studied in order to determine the influence of antibiotic ciprofloxacin concentration on the E. coli migration.
关键词[WOS]: PAPER-BASED MICROFLUIDICS ;  ELECTROPORATION CHIP ;  LOW-COST ;  DEVICES ;  FABRICATION ;  SYSTEMS ;  POLY(DIMETHYLSILOXANE) ;  CELLS ;  VISUALIZATION ;  PDMS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000281614900021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103441
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Beijing 11603, Peoples R China
2.Hong Kong Univ Sci & Technol, Dept Phys, Kowloon, Hong Kong, Peoples R China
3.Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China
4.Hong Kong Univ Sci & Technol, KAUST HKUST Micro Nanofluid Joint Lab, Kowloon, Hong Kong, Peoples R China

Recommended Citation:
Gong, Xiuqing,Yi, Xin,Xiao, Kang,et al. Wax-bonding 3D microfluidic chips[J]. LAB ON A CHIP,2010,10(19):2622-2627.
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