DICP OpenIR
Wax-bonding 3D microfluidic chips
Gong, Xiuqing2; Yi, Xin2; Xiao, Kang3; Li, Shunbo2; Kodzius, Rimantas4; Qin, Jianhua1; Wen, Weijia2,4; Weijia Wen
Source PublicationLAB ON A CHIP
2010
DOI10.1039/c004744a
Volume10Issue:19Pages:2622-2627
Indexed BySCI
SubtypeArticle
Department18
Funding Project1807
Contribution Rank4;6
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS KeywordPAPER-BASED MICROFLUIDICS ; ELECTROPORATION CHIP ; LOW-COST ; DEVICES ; FABRICATION ; SYSTEMS ; POLY(DIMETHYLSILOXANE) ; CELLS ; VISUALIZATION ; PDMS
AbstractWe report a simple, low-cost and detachable microfluidic chip incorporating easily accessible paper, glass slides or other polymer films as the chip materials along with adhesive wax as the recycling bonding material. We use a laser to cut through the paper or film to form patterns and then sandwich the paper and film between glass sheets or polymer membranes. The hot-melt adhesive wax can realize bridge bonding between various materials, for example, paper, polymethylmethacrylate (PMMA) film, glass sheets, or metal plate. The bonding process is reversible and the wax is reusable through a melting and cooling process. With this process, a three-dimensional (3D) microfluidic chip is achievable by vacuating and venting the chip in a hot-water bath. To study the biocompatibility and applicability of the wax-based microfluidic chip, we tested the PCR compatibility with the chip materials first. Then we applied the wax-paper based microfluidic chip to HeLa cell electroporation (EP). Subsequently, a prototype of a 5-layer 3D chip was fabricated by multilayer wax bonding. To check the sealing ability and the durability of the chip, green fluorescence protein (GFP) recombinant Escherichia coli (E. coli) bacteria were cultured, with which the chemotaxis of E. coli was studied in order to determine the influence of antibiotic ciprofloxacin concentration on the E. coli migration.
Language英语
URL查看原文
WOS IDWOS:000281614900021
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/103441
Collection中国科学院大连化学物理研究所
Corresponding AuthorWeijia Wen
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Beijing 11603, Peoples R China
2.Hong Kong Univ Sci & Technol, Dept Phys, Kowloon, Hong Kong, Peoples R China
3.Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China
4.Hong Kong Univ Sci & Technol, KAUST HKUST Micro Nanofluid Joint Lab, Kowloon, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Gong, Xiuqing,Yi, Xin,Xiao, Kang,et al. Wax-bonding 3D microfluidic chips[J]. LAB ON A CHIP,2010,10(19):2622-2627.
APA Gong, Xiuqing.,Yi, Xin.,Xiao, Kang.,Li, Shunbo.,Kodzius, Rimantas.,...&Weijia Wen.(2010).Wax-bonding 3D microfluidic chips.LAB ON A CHIP,10(19),2622-2627.
MLA Gong, Xiuqing,et al."Wax-bonding 3D microfluidic chips".LAB ON A CHIP 10.19(2010):2622-2627.
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