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题名: Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography
作者: Liu, Jinxiang1, 2;  Deng, Qiliang3;  Yang, Kaiguang1;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: HPLC ;  Molecular imprinting ;  Polyacrylamide monolith ;  Protein recognition
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2010-09-01
DOI: 10.1002/jssc.201000350
卷: 33, 期:17-18, 页:2757-2761
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Macroporous cytochrome c (cyc)-imprinted monolithic polyarylamide columns were prepared, and applied for the template protein recognition by HPLC. With cyc (18.8 mg) as template, the imprinted monolithic materials were in situ polymerized in an HPLC column tube, with methacrylamide (450 mg), methacrylic acid (15.8 mg), piperazine diacrylamide (720 mg) and ammonium sulfate (390 mg) dissolved in 5 mL of phosphate buffer (pH 7.4), initiated by ammonium persulfate and TEMED. After the reaction, cyc was removed with acetic acid (10%, v/v) containing 10% w/v SDS. The non-imprinted monolithic column was prepared under the same procedure except without cyc. Retention of cyc and its competitive protein, lysozyme (lys), on molecular-imprinting polymer (MIP) and non-imprinted polymer columns was studied. When the pH value of mobile phase was 4.0, on MIP column, the retention factors of cyc and lys were 2.0 and 1.3, respectively. However, those on non-imprinted polymer column were very similar, both as 1.1. Even in competitive environment, a mixture of cyc and lys could be separated on MIP column under gradient elution, with resolution as 1.2. These results indicate that protein-imprinted monolithic polymer columns could offer obvious affinity and specific recognition to the template protein.
关键词[WOS]: STATIONARY PHASES ;  ANTIBODIES ;  DELIVERY ;  BLOOD
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000282612900025
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103559
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.China Acad Sci, Grad Univ, Beijing, Peoples R China
3.Tianjin Univ Sci & Technol, Coll Sci, Tianjin, Peoples R China

Recommended Citation:
Liu, Jinxiang,Deng, Qiliang,Yang, Kaiguang,et al. Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(17-18):2757-2761.
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