DICP OpenIR
Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography
Liu, Jinxiang1,2; Deng, Qiliang3; Yang, Kaiguang1; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
关键词Hplc Molecular Imprinting Polyacrylamide Monolith Protein Recognition
刊名JOURNAL OF SEPARATION SCIENCE
2010-09-01
DOI10.1002/jssc.201000350
33期:17-18页:2757-2761
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]STATIONARY PHASES ; ANTIBODIES ; DELIVERY ; BLOOD
英文摘要Macroporous cytochrome c (cyc)-imprinted monolithic polyarylamide columns were prepared, and applied for the template protein recognition by HPLC. With cyc (18.8 mg) as template, the imprinted monolithic materials were in situ polymerized in an HPLC column tube, with methacrylamide (450 mg), methacrylic acid (15.8 mg), piperazine diacrylamide (720 mg) and ammonium sulfate (390 mg) dissolved in 5 mL of phosphate buffer (pH 7.4), initiated by ammonium persulfate and TEMED. After the reaction, cyc was removed with acetic acid (10%, v/v) containing 10% w/v SDS. The non-imprinted monolithic column was prepared under the same procedure except without cyc. Retention of cyc and its competitive protein, lysozyme (lys), on molecular-imprinting polymer (MIP) and non-imprinted polymer columns was studied. When the pH value of mobile phase was 4.0, on MIP column, the retention factors of cyc and lys were 2.0 and 1.3, respectively. However, those on non-imprinted polymer column were very similar, both as 1.1. Even in competitive environment, a mixture of cyc and lys could be separated on MIP column under gradient elution, with resolution as 1.2. These results indicate that protein-imprinted monolithic polymer columns could offer obvious affinity and specific recognition to the template protein.
语种英语
原文出处查看原文
WOS记录号WOS:000282612900025
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103559
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.China Acad Sci, Grad Univ, Beijing, Peoples R China
3.Tianjin Univ Sci & Technol, Coll Sci, Tianjin, Peoples R China
推荐引用方式
GB/T 7714
Liu, Jinxiang,Deng, Qiliang,Yang, Kaiguang,et al. Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(17-18):2757-2761.
APA Liu, Jinxiang.,Deng, Qiliang.,Yang, Kaiguang.,Zhang, Lihua.,Liang, Zhen.,...&张丽华.(2010).Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography.JOURNAL OF SEPARATION SCIENCE,33(17-18),2757-2761.
MLA Liu, Jinxiang,et al."Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography".JOURNAL OF SEPARATION SCIENCE 33.17-18(2010):2757-2761.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Jinxiang]的文章
[Deng, Qiliang]的文章
[Yang, Kaiguang]的文章
百度学术
百度学术中相似的文章
[Liu, Jinxiang]的文章
[Deng, Qiliang]的文章
[Yang, Kaiguang]的文章
必应学术
必应学术中相似的文章
[Liu, Jinxiang]的文章
[Deng, Qiliang]的文章
[Yang, Kaiguang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。