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题名: Protein separation using free-flow electrophoresis microchip etched in a single step
作者: Wang, Pingli1, 2;  Zhang, Lihua1;  Shan, Yichu1;  Cong, Yongzheng1, 2;  Liang, Yu1, 2;  Han, Bin1, 3;  Liang, Zhen1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Fluorescence image ;  Free-flow electrophoresis ;  Microchip ;  One-step etching ;  Protein separation
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2010-07-01
DOI: 10.1002/jssc.201000162
卷: 33, 期:13, 页:2039-2044
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A one-step etching method was developed to fabricate glass free-flow electrophoresis microchips with a rectangle separation microchamber (42 mm-long, 23 mm-wide and 28 mu m-deep), in which two glass bridges (0.5 mm-wide) were made simultaneously to prevent bubbles formed by electrolysis near the Pt electrode from entering the separation chamber. By microchip free-flow zone electrophoresis, with 200 V voltage applied, the baseline separation of three FITC labeled proteins, ribonuclease B, myoglobin and beta-lactoglobulin, was achieved, with resolution over 1.78. Furthermore, with 2.5 mIvl Na(2)SO(4) added into the electrode buffer to form higher electrical field strength across separation microchamber than electrode compartments, similar resolution of samples was achieved with the applied voltage decreased to 75 V, which could obviously decrease Joule heat during continuous separation. All these results demonstrate that the free-flow electrophoresis microchip fabricated by one-step etching method is suitable for the continuous separation of proteins, which might become an effective pre-fractionation method for proteome study.
关键词[WOS]: MASS-SPECTROMETRY ;  DEVICE ;  CHIP ;  PROTEOMICS ;  RESOLUTION ;  SPEED
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000280163900022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103567
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China

Recommended Citation:
Wang, Pingli,Zhang, Lihua,Shan, Yichu,et al. Protein separation using free-flow electrophoresis microchip etched in a single step[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(13):2039-2044.
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