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Protein separation using free-flow electrophoresis microchip etched in a single step
Wang, Pingli1,2; Zhang, Lihua1; Shan, Yichu1; Cong, Yongzheng1,2; Liang, Yu1,2; Han, Bin1,3; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
KeywordFluorescence Image Free-flow Electrophoresis Microchip One-step Etching Protein Separation
Source PublicationJOURNAL OF SEPARATION SCIENCE
2010-07-01
DOI10.1002/jssc.201000162
Volume33Issue:13Pages:2039-2044
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMASS-SPECTROMETRY ; DEVICE ; CHIP ; PROTEOMICS ; RESOLUTION ; SPEED
AbstractA one-step etching method was developed to fabricate glass free-flow electrophoresis microchips with a rectangle separation microchamber (42 mm-long, 23 mm-wide and 28 mu m-deep), in which two glass bridges (0.5 mm-wide) were made simultaneously to prevent bubbles formed by electrolysis near the Pt electrode from entering the separation chamber. By microchip free-flow zone electrophoresis, with 200 V voltage applied, the baseline separation of three FITC labeled proteins, ribonuclease B, myoglobin and beta-lactoglobulin, was achieved, with resolution over 1.78. Furthermore, with 2.5 mIvl Na(2)SO(4) added into the electrode buffer to form higher electrical field strength across separation microchamber than electrode compartments, similar resolution of samples was achieved with the applied voltage decreased to 75 V, which could obviously decrease Joule heat during continuous separation. All these results demonstrate that the free-flow electrophoresis microchip fabricated by one-step etching method is suitable for the continuous separation of proteins, which might become an effective pre-fractionation method for proteome study.
Language英语
URL查看原文
WOS IDWOS:000280163900022
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/103567
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Wang, Pingli,Zhang, Lihua,Shan, Yichu,et al. Protein separation using free-flow electrophoresis microchip etched in a single step[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(13):2039-2044.
APA Wang, Pingli.,Zhang, Lihua.,Shan, Yichu.,Cong, Yongzheng.,Liang, Yu.,...&张丽华.(2010).Protein separation using free-flow electrophoresis microchip etched in a single step.JOURNAL OF SEPARATION SCIENCE,33(13),2039-2044.
MLA Wang, Pingli,et al."Protein separation using free-flow electrophoresis microchip etched in a single step".JOURNAL OF SEPARATION SCIENCE 33.13(2010):2039-2044.
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