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Protein separation using free-flow electrophoresis microchip etched in a single step
Wang, Pingli1,2; Zhang, Lihua1; Shan, Yichu1; Cong, Yongzheng1,2; Liang, Yu1,2; Han, Bin1,3; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
关键词Fluorescence Image Free-flow Electrophoresis Microchip One-step Etching Protein Separation
刊名JOURNAL OF SEPARATION SCIENCE
2010-07-01
DOI10.1002/jssc.201000162
33期:13页:2039-2044
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MASS-SPECTROMETRY ; DEVICE ; CHIP ; PROTEOMICS ; RESOLUTION ; SPEED
英文摘要A one-step etching method was developed to fabricate glass free-flow electrophoresis microchips with a rectangle separation microchamber (42 mm-long, 23 mm-wide and 28 mu m-deep), in which two glass bridges (0.5 mm-wide) were made simultaneously to prevent bubbles formed by electrolysis near the Pt electrode from entering the separation chamber. By microchip free-flow zone electrophoresis, with 200 V voltage applied, the baseline separation of three FITC labeled proteins, ribonuclease B, myoglobin and beta-lactoglobulin, was achieved, with resolution over 1.78. Furthermore, with 2.5 mIvl Na(2)SO(4) added into the electrode buffer to form higher electrical field strength across separation microchamber than electrode compartments, similar resolution of samples was achieved with the applied voltage decreased to 75 V, which could obviously decrease Joule heat during continuous separation. All these results demonstrate that the free-flow electrophoresis microchip fabricated by one-step etching method is suitable for the continuous separation of proteins, which might become an effective pre-fractionation method for proteome study.
语种英语
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WOS记录号WOS:000280163900022
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被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103567
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China
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Wang, Pingli,Zhang, Lihua,Shan, Yichu,et al. Protein separation using free-flow electrophoresis microchip etched in a single step[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(13):2039-2044.
APA Wang, Pingli.,Zhang, Lihua.,Shan, Yichu.,Cong, Yongzheng.,Liang, Yu.,...&张丽华.(2010).Protein separation using free-flow electrophoresis microchip etched in a single step.JOURNAL OF SEPARATION SCIENCE,33(13),2039-2044.
MLA Wang, Pingli,et al."Protein separation using free-flow electrophoresis microchip etched in a single step".JOURNAL OF SEPARATION SCIENCE 33.13(2010):2039-2044.
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