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题名: Recent advances in proteolysis and peptide/protein separation by chromatographic strategies
作者: Zhang XiangMin1, 2;  Liu BaoHong1, 2;  Zhang LiHua3;  Zou HanFa3;  Cao Jing1, 2;  Gao MingXia1, 2;  Tang Jia1, 2;  Liu Yun1, 2;  Yang PengYuan1, 2;  Zhang YuKui3
通讯作者: 刘云 ;  张玉奎
关键词: proteolysis ;  enzymatic digestion ;  chromatographic separation ;  peptide enrichment ;  mass spectrometry
刊名: SCIENCE CHINA-CHEMISTRY
发表日期: 2010-04-01
DOI: 10.1007/s11426-010-0135-7
卷: 53, 期:4, 页:685-694
收录类别: SCI
文章类型: Review
部门归属: 18
项目归属: 1810
产权排名: 3;2
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chromatographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  MULTIDIMENSIONAL LIQUID-CHROMATOGRAPHY ;  HIGHLY EFFICIENT PROTEOLYSIS ;  MONOLITHIC ENZYMATIC MICROREACTOR ;  IMMOBILIZED TRYPSIN MICROREACTOR ;  PEPTIDE-MAPPING ANALYSIS ;  EXCHANGE TRAP COLUMN ;  LC-ESI-MS/MS ;  PROTEOME ANALYSIS ;  HUMAN PLASMA
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000278263400002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103569
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
2.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhang XiangMin,Liu BaoHong,Zhang LiHua,et al. Recent advances in proteolysis and peptide/protein separation by chromatographic strategies[J]. SCIENCE CHINA-CHEMISTRY,2010,53(4):685-694.
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