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Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers
Zhang, Wei1; Qin, Lei1; Chen, Run-Run1; He, Xi-Wen1; Li, Wen-You1; Zhang, Yu-Kui1,2; Li WY(李文友)
关键词Surface Molecular Imprinting Beta-cyclodextrin Bovine Hemoglobin Recognition
刊名APPLIED SURFACE SCIENCE
2010-02-15
DOI10.1016/j.apsusc.2009.11.064
256期:9页:3000-3005
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名6;2
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Physical ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
研究领域[WOS]Chemistry ; Materials Science ; Physics
关键词[WOS]BOVINE SERUM-ALBUMIN ; SUBMICROMETER PARTICLES ; SELECTIVE RECOGNITION ; AQUEOUS-MEDIA ; SURFACE ; GEL ; ANTIBODIES ; SEPARATION ; PEPTIDES ; BEADS
英文摘要A novel protein imprinted polymer was prepared using acryloyl-beta-cyclodextrin (beta-CD) and acrylamide as monomers on the surface of silica gel. The bovine hemoglobin was used as template and beta-CD was allowed to self-assemble with the template protein through hydrogen bonding and hydrophobic interaction. Polymerization was carried out in the presence of acrylamide as an assistant monomer, which resulted in a novel protein imprinted polymer. After removing the template, imprinted cavities with the shape and spatial distribution of functional groups were formed. Bovine serum albumin (BSA) cytochrome c (Cyt) and lysozyme (Lyz) were employed as non-template proteins to test the imprinting effect and the specific binding of bovine hemoglobin to the polymer. The results of the adsorption experiments indicated that such protein imprinted polymer, which was synthesized with beta-CD and acrylamide as monomers, could selectively recognize the template protein. (C) 2009 Elsevier B. V. All rights reserved.
语种英语
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WOS记录号WOS:000274153800055
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103577
专题中国科学院大连化学物理研究所
通讯作者Li WY(李文友)
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
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Zhang, Wei,Qin, Lei,Chen, Run-Run,et al. Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers[J]. APPLIED SURFACE SCIENCE,2010,256(9):3000-3005.
APA Zhang, Wei.,Qin, Lei.,Chen, Run-Run.,He, Xi-Wen.,Li, Wen-You.,...&李文友.(2010).Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers.APPLIED SURFACE SCIENCE,256(9),3000-3005.
MLA Zhang, Wei,et al."Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers".APPLIED SURFACE SCIENCE 256.9(2010):3000-3005.
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