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题名: Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers
作者: Zhang, Wei1;  Qin, Lei1;  Chen, Run-Run1;  He, Xi-Wen1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2
通讯作者: 李文友
关键词: Surface molecular imprinting ;  beta-Cyclodextrin ;  Bovine hemoglobin ;  Recognition
刊名: APPLIED SURFACE SCIENCE
发表日期: 2010-02-15
DOI: 10.1016/j.apsusc.2009.11.064
卷: 256, 期:9, 页:3000-3005
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 6;2
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Physical ;  Materials Science, Coatings & Films ;  Physics, Applied ;  Physics, Condensed Matter
研究领域[WOS]: Chemistry ;  Materials Science ;  Physics
英文摘要: A novel protein imprinted polymer was prepared using acryloyl-beta-cyclodextrin (beta-CD) and acrylamide as monomers on the surface of silica gel. The bovine hemoglobin was used as template and beta-CD was allowed to self-assemble with the template protein through hydrogen bonding and hydrophobic interaction. Polymerization was carried out in the presence of acrylamide as an assistant monomer, which resulted in a novel protein imprinted polymer. After removing the template, imprinted cavities with the shape and spatial distribution of functional groups were formed. Bovine serum albumin (BSA) cytochrome c (Cyt) and lysozyme (Lyz) were employed as non-template proteins to test the imprinting effect and the specific binding of bovine hemoglobin to the polymer. The results of the adsorption experiments indicated that such protein imprinted polymer, which was synthesized with beta-CD and acrylamide as monomers, could selectively recognize the template protein. (C) 2009 Elsevier B. V. All rights reserved.
关键词[WOS]: BOVINE SERUM-ALBUMIN ;  SUBMICROMETER PARTICLES ;  SELECTIVE RECOGNITION ;  AQUEOUS-MEDIA ;  SURFACE ;  GEL ;  ANTIBODIES ;  SEPARATION ;  PEPTIDES ;  BEADS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000274153800055
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103577
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zhang, Wei,Qin, Lei,Chen, Run-Run,et al. Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers[J]. APPLIED SURFACE SCIENCE,2010,256(9):3000-3005.
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