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Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers
Zhang, Wei1; Qin, Lei1; Chen, Run-Run1; He, Xi-Wen1; Li, Wen-You1; Zhang, Yu-Kui1,2; Li WY(李文友)
KeywordSurface Molecular Imprinting Beta-cyclodextrin Bovine Hemoglobin Recognition
Source PublicationAPPLIED SURFACE SCIENCE
2010-02-15
DOI10.1016/j.apsusc.2009.11.064
Volume256Issue:9Pages:3000-3005
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank6;2
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Physical ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
WOS Research AreaChemistry ; Materials Science ; Physics
WOS KeywordBOVINE SERUM-ALBUMIN ; SUBMICROMETER PARTICLES ; SELECTIVE RECOGNITION ; AQUEOUS-MEDIA ; SURFACE ; GEL ; ANTIBODIES ; SEPARATION ; PEPTIDES ; BEADS
AbstractA novel protein imprinted polymer was prepared using acryloyl-beta-cyclodextrin (beta-CD) and acrylamide as monomers on the surface of silica gel. The bovine hemoglobin was used as template and beta-CD was allowed to self-assemble with the template protein through hydrogen bonding and hydrophobic interaction. Polymerization was carried out in the presence of acrylamide as an assistant monomer, which resulted in a novel protein imprinted polymer. After removing the template, imprinted cavities with the shape and spatial distribution of functional groups were formed. Bovine serum albumin (BSA) cytochrome c (Cyt) and lysozyme (Lyz) were employed as non-template proteins to test the imprinting effect and the specific binding of bovine hemoglobin to the polymer. The results of the adsorption experiments indicated that such protein imprinted polymer, which was synthesized with beta-CD and acrylamide as monomers, could selectively recognize the template protein. (C) 2009 Elsevier B. V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000274153800055
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/103577
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi WY(李文友)
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Wei,Qin, Lei,Chen, Run-Run,et al. Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers[J]. APPLIED SURFACE SCIENCE,2010,256(9):3000-3005.
APA Zhang, Wei.,Qin, Lei.,Chen, Run-Run.,He, Xi-Wen.,Li, Wen-You.,...&李文友.(2010).Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers.APPLIED SURFACE SCIENCE,256(9),3000-3005.
MLA Zhang, Wei,et al."Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers".APPLIED SURFACE SCIENCE 256.9(2010):3000-3005.
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