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题名: Magnetic Silica-Coated Sub-Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood
作者: Zhang, Min1;  Cheng, Dan1;  He, Xiwen1;  Chen, Langxing1;  Zhang, Yukui1, 2
通讯作者: 陈朗星
关键词: bovine hemoglobin ;  immobilization ;  magnetic properties ;  removal ;  silanes
刊名: CHEMISTRY-AN ASIAN JOURNAL
发表日期: 2010
DOI: 10.1002/asia.200900463
卷: 5, 期:6, 页:1332-1340
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 5;2
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Magnetic silica-coated magnetite (Fe(3)O(4)) sub-microspheres with immobilized metal-affinity ligands are prepared for protein adsorption. First, magnetite sub-microspheres were synthesized by a hydrothermal method. Then silica was coated on the surface of Fe(3)O(4) particles using a sol gel method to obtain magnetic silica sub-microspheres with core-shell morphology. Next, the trichloro(4-chloromethyl-phenyl) silane was immobilized on them, reacted with iminodiacetic acid (IDA), and charged with Cu(2+). The obtained magnetic silica sub-microspheres with immobilized Cu(2+) were applied for the absorption of bovine hemoglobin (BHb) and the removal of BHb from bovine blood. The size, morphology, and magnetic properties of the resulting magnetic micro(nano) spheres were investigated by using scanning microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and a vibrating sample magnetometer (VSM). The measurements showed that the magnetic sub-micro-spheres are spherical in shape, very uniform in size with a core-shell, and are almost superparamagnetic. The saturation magnetization of silica-coated magnetite (Fe(3)O(4)) sub-microspheres reached about 33 emu g(-1). Protein adsorption results showed that the sub-microspheres had a high adsorption capacity for BHb (418.6 mg g(-1)), low nonspecific adsorption, and good removal of BHb from bovine blood. This opens a novel route for future applications in removing abundant proteins in proteomic analysis.
关键词[WOS]: TOF-MS ANALYSIS ;  IONIZATION MASS-SPECTROMETRY ;  LOW-CONCENTRATION PEPTIDES ;  HISTIDINE-TAGGED PROTEINS ;  IRON-OXIDE NANOPARTICLES ;  FACILE SYNTHESIS ;  AFFINITY-CHROMATOGRAPHY ;  POLYMER NANOPARTICLES ;  ENRICHMENT ;  SHELL
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000278840200011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103583
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Zhang, Min,Cheng, Dan,He, Xiwen,et al. Magnetic Silica-Coated Sub-Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood[J]. CHEMISTRY-AN ASIAN JOURNAL,2010,5(6):1332-1340.
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