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Magnetic Silica-Coated Sub-Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood
Zhang, Min1; Cheng, Dan1; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,2; Chen LX(陈朗星)
关键词Bovine Hemoglobin Immobilization Magnetic Properties Removal Silanes
刊名CHEMISTRY-AN ASIAN JOURNAL
2010
DOI10.1002/asia.200900463
5期:6页:1332-1340
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名5;2
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]TOF-MS ANALYSIS ; IONIZATION MASS-SPECTROMETRY ; LOW-CONCENTRATION PEPTIDES ; HISTIDINE-TAGGED PROTEINS ; IRON-OXIDE NANOPARTICLES ; FACILE SYNTHESIS ; AFFINITY-CHROMATOGRAPHY ; POLYMER NANOPARTICLES ; ENRICHMENT ; SHELL
英文摘要Magnetic silica-coated magnetite (Fe(3)O(4)) sub-microspheres with immobilized metal-affinity ligands are prepared for protein adsorption. First, magnetite sub-microspheres were synthesized by a hydrothermal method. Then silica was coated on the surface of Fe(3)O(4) particles using a sol gel method to obtain magnetic silica sub-microspheres with core-shell morphology. Next, the trichloro(4-chloromethyl-phenyl) silane was immobilized on them, reacted with iminodiacetic acid (IDA), and charged with Cu(2+). The obtained magnetic silica sub-microspheres with immobilized Cu(2+) were applied for the absorption of bovine hemoglobin (BHb) and the removal of BHb from bovine blood. The size, morphology, and magnetic properties of the resulting magnetic micro(nano) spheres were investigated by using scanning microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and a vibrating sample magnetometer (VSM). The measurements showed that the magnetic sub-micro-spheres are spherical in shape, very uniform in size with a core-shell, and are almost superparamagnetic. The saturation magnetization of silica-coated magnetite (Fe(3)O(4)) sub-microspheres reached about 33 emu g(-1). Protein adsorption results showed that the sub-microspheres had a high adsorption capacity for BHb (418.6 mg g(-1)), low nonspecific adsorption, and good removal of BHb from bovine blood. This opens a novel route for future applications in removing abundant proteins in proteomic analysis.
语种英语
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WOS记录号WOS:000278840200011
引用统计
被引频次:54[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103583
专题中国科学院大连化学物理研究所
通讯作者Chen LX(陈朗星)
作者单位1.Nankai Univ, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Zhang, Min,Cheng, Dan,He, Xiwen,et al. Magnetic Silica-Coated Sub-Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood[J]. CHEMISTRY-AN ASIAN JOURNAL,2010,5(6):1332-1340.
APA Zhang, Min,Cheng, Dan,He, Xiwen,Chen, Langxing,Zhang, Yukui,&陈朗星.(2010).Magnetic Silica-Coated Sub-Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood.CHEMISTRY-AN ASIAN JOURNAL,5(6),1332-1340.
MLA Zhang, Min,et al."Magnetic Silica-Coated Sub-Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood".CHEMISTRY-AN ASIAN JOURNAL 5.6(2010):1332-1340.
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