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Development of Identification for Post-Translational Modifications in Histones by Mass Spectrometry Based Proteomics
Chen Ying1; Zhang Kai1; He Xiwen1; Zhang Yukui1,2
KeywordHistone Post-translational Modifications (Ptms) Mass Spectrometry High Prerormance Liquid Chromatography (Hplc)
Source PublicationPROGRESS IN CHEMISTRY
2010-04-01
Volume22Issue:4Pages:713-719
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank4;2
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordELECTRON-TRANSFER DISSOCIATION ; H3 LYSINE-56 ACETYLATION ; TOP-DOWN PROTEOMICS ; H1 ISOFORMS ; CELL-CYCLE ; CHICKEN ERYTHROCYTES ; DYNAMIC CHANGES ; GLOBAL CHANGES ; CORE HISTONES ; AMINO-ACIDS
AbstractIn eukaryotic cells, chromosomal DNA is packaged into a compact structure, chromatin, with the help of four core histones. The fundamental repeating unit of chromatin is the nucleosome, which contains an octamer of core histones, around 147 base pairs of DNA are wrapped. Post-translational modifications (PTMs) in histones are generally considered to be a major group of epigenetic marks. Accordingly, because PTMs alter the properties of histones, hence affect chromatin structure and function and regulate the gene expression. Therefore identification of histone PTMs is the key to discover histone codes. At present, mass spectrometry has become a powerful analysis tool. In this paper we introduced the novel development of the strategies based on mass spectrometry ("bottom-up" and "top-down") in mapping histone PTMs, fragment dissociation technologies such as CID, ECD and ETD. We also reported the application of mass spectrometry in addressing histone PTMs sites, identifying histone variants and quantifying differential expression of these PTMs in cells.
Language英语
URL查看原文
WOS IDWOS:000276929300018
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/103585
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang Kai
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Chen Ying,Zhang Kai,He Xiwen,et al. Development of Identification for Post-Translational Modifications in Histones by Mass Spectrometry Based Proteomics[J]. PROGRESS IN CHEMISTRY,2010,22(4):713-719.
APA Chen Ying,Zhang Kai,He Xiwen,&Zhang Yukui.(2010).Development of Identification for Post-Translational Modifications in Histones by Mass Spectrometry Based Proteomics.PROGRESS IN CHEMISTRY,22(4),713-719.
MLA Chen Ying,et al."Development of Identification for Post-Translational Modifications in Histones by Mass Spectrometry Based Proteomics".PROGRESS IN CHEMISTRY 22.4(2010):713-719.
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