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题名: Development of Identification for Post-Translational Modifications in Histones by Mass Spectrometry Based Proteomics
作者: Chen Ying1;  Zhang Kai1;  He Xiwen1;  Zhang Yukui1, 2
通讯作者: Zhang Kai
关键词: histone ;  post-translational modifications (PTMs) ;  mass spectrometry ;  high prerormance liquid chromatography (HPLC)
刊名: PROGRESS IN CHEMISTRY
发表日期: 2010-04-01
卷: 22, 期:4, 页:713-719
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 4;2
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: In eukaryotic cells, chromosomal DNA is packaged into a compact structure, chromatin, with the help of four core histones. The fundamental repeating unit of chromatin is the nucleosome, which contains an octamer of core histones, around 147 base pairs of DNA are wrapped. Post-translational modifications (PTMs) in histones are generally considered to be a major group of epigenetic marks. Accordingly, because PTMs alter the properties of histones, hence affect chromatin structure and function and regulate the gene expression. Therefore identification of histone PTMs is the key to discover histone codes. At present, mass spectrometry has become a powerful analysis tool. In this paper we introduced the novel development of the strategies based on mass spectrometry ("bottom-up" and "top-down") in mapping histone PTMs, fragment dissociation technologies such as CID, ECD and ETD. We also reported the application of mass spectrometry in addressing histone PTMs sites, identifying histone variants and quantifying differential expression of these PTMs in cells.
关键词[WOS]: ELECTRON-TRANSFER DISSOCIATION ;  H3 LYSINE-56 ACETYLATION ;  TOP-DOWN PROTEOMICS ;  H1 ISOFORMS ;  CELL-CYCLE ;  CHICKEN ERYTHROCYTES ;  DYNAMIC CHANGES ;  GLOBAL CHANGES ;  CORE HISTONES ;  AMINO-ACIDS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000276929300018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103585
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Chen Ying,Zhang Kai,He Xiwen,et al. Development of Identification for Post-Translational Modifications in Histones by Mass Spectrometry Based Proteomics[J]. PROGRESS IN CHEMISTRY,2010,22(4):713-719.
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