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题名: Microcapsules Embedded with Three-Dimensional Fibrous Scaffolds for Cell Culture and Tissue Engineering
作者: Huang, Xiaobo1, 2;  Wang, Jianzheng1, 2;  Xie, Hongguo1, 2;  Zhang, Ying1;  Wang, Wei1;  Yu, Weiting1;  Liu, Yang1;  Ma, Xiaojun1
通讯作者: 马小军
刊名: TISSUE ENGINEERING PART C-METHODS
发表日期: 2010-10-01
DOI: 10.1089/ten.tec.2009.0545
卷: 16, 期:5, 页:1023-1032
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell & Tissue Engineering ;  Biotechnology & Applied Microbiology ;  Cell Biology
研究领域[WOS]: Cell Biology ;  Biotechnology & Applied Microbiology
英文摘要: Aggregating into multicellular spheroids within alginate-poly-l-lysine-alginate (APA) microcapsules is important in maintaining the cellular viability and specific functions. However, in the absence of a vascular network, cells in the core of large-sized spheroids are gradually necrotic because of oxygen transfer limitations. In this study, a novel APA microcapsule embedded with three-dimensional fibrous scaffolds (called APA-FS) was proposed to eliminate cellular necrosis by regulating cells to form multi-small spheroids. HepG2 cells were embedded within the APA-FS to form spheroids and the state of these spheroids was evaluated via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide assay, glucose/lactate metabolism, live/dead staining, and hematoxylin and eosin staining. Comparing with the conventional APA microcapsules, the cells within APA-FS organized into multi-small spheroids. The size of these spheroids depended on the concentration of fibrous scaffolds embedded within the microcapsules. In the APA-FS embedded with 5% (v/v) fibrous scaffolds, the average size of cellular spheroids was controlled below 100 mu m and the cellular viability was increased by 50% than the control. The results of live/dead staining and hematoxylin and eosin staining showed that the improved cellular viability might be attributed to the decreased necrosis in the core of these spheroids. The improved viability of cells demonstrated the efficiency of this technology. These findings implied that this system might provide a more suitable culture environment for a variety of tissue engineering applications.
关键词[WOS]: HEPATOCYTE SPHEROIDS ;  ALGINATE SCAFFOLDS ;  APA MICROCAPSULE ;  MASS-TRANSFER ;  TUMOR ;  MODEL ;  VIABILITY ;  LIVER ;  PROLIFERATION ;  METABOLISM
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000282223800022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103651
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Huang, Xiaobo,Wang, Jianzheng,Xie, Hongguo,et al. Microcapsules Embedded with Three-Dimensional Fibrous Scaffolds for Cell Culture and Tissue Engineering[J]. TISSUE ENGINEERING PART C-METHODS,2010,16(5):1023-1032.
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