DICP OpenIR
学科主题物理化学
Characterization of Chitosan Matrices by Confocal Laser Scanning Microscopy
Jie YB(解玉冰); Yu WT(于炜婷); Kang XH(康西海); Ren DW(任东文); Ma XJ(马小军); Kniss; DouglasA; Yuan Q(袁权); Xie Yubing
会议名称Tissue Engineering Society International 6th International Conference
会议日期2003-12-10
2003-12-10
会议地点美国
其他题名共聚焦激光扫描显微镜用于壳聚糖基质的表征
页码7/1
部门归属十八室
英文摘要Biodegradable polymers are in great demand in tissue engineering. Chitosan is one of the promising natural biopolymers to mimic extracellular matrix (ECM) for tissue repair and regeneration. Currently, porous chitosan matrices have been fabricated using freezing-dry technique and characterized by Scanning Electronic Microscopy (SEM). However, SEM only shows the surface structure of dried materials. It is of paramount importance to develop a method to reveal the real network structure inside the scaffolds. In this study, Fluorescein isothiocyanate (FITC)-labeled chitosan matrices were fabricated and characterized using Confocal Laser Scanning Microscopy (CLSM). The effects of molecular weight and concentration of chitosan as well as the freezing temperature on the three-dimensional (3-D) structure of chitosan matrices were investigated. The real structure of the chitosan matrix was revealed by CLSM without any treatment, which was composed of a network with open-pores interacted in 3-D. The pose size decreased with an increase in molecular weight or concentration of chitosan, and with a decrease in freezing temperature. The structure of matrices showed a fiber-like orientation at low concentration and at -20°C, while it was mesh-like at high concentration or at -80°C. The CLSM provides a useful tool for the study of the structure of chitosan matrices and the interaction of cells with chitosan matrices.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/111454
专题中国科学院大连化学物理研究所
通讯作者Xie Yubing
推荐引用方式
GB/T 7714
Jie YB,Yu WT,Kang XH,et al. Characterization of Chitosan Matrices by Confocal Laser Scanning Microscopy[C],2003:7/1.
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