Subject Area物理化学
Regulatin of photobiological hydrogen production in Platymonas subcordiformis by carbonylcyanide m-chlorophenylhydrazone
Ran CQ(冉春秋); Chen ZA(陈兆安); Yu XJ(虞星炬); Jin MF(金美芳); Zhang W(张卫)
Conference Name6th Asia-Pacific Marine Biotechnology Conference
Conference Date2004-9-27
Conference Place中国
Alternative Title羰基氰化物间氯苯腙促进海洋亚心型扁藻光照产氢过程的研究
AbstractThe marine green alga Platymonas subcordiformis can produce a significant amount of hydrogen under anaerobic condition and the addition of a proton gradient uncoupler, carbonylcyanide-m-Chlorophenylhydrazone(CCCP). About 7.54ml H2 was produced at 3x106 cells/ml for 12h in a 760ml photobioreactor when 15μM CCCP was added at the start of light illumination. During the stage of hydrogen evolution, the extracellular pH and the photochemical activity of PSII reduced from 7.62 to 7.49 and from 0.342 to 0.002, respectively. The system holds at a very low oxygen level. These results indicated that the proton gradient uncoupler CCCP can facilitate the proton transfer across the thylakoid membrane, thus enhance the accessibility of H+ to hydrogenase, and keep anaerobic condition during hydrogen production through markedly inhibition of the PSII activity.
Document Type会议论文
Corresponding AuthorZhang W(张卫)
Recommended Citation
GB/T 7714
Ran CQ,Chen ZA,Yu XJ,et al. Regulatin of photobiological hydrogen production in Platymonas subcordiformis by carbonylcyanide m-chlorophenylhydrazone[C],2004:100/1.
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