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学科主题: 物理化学
题名: Mucin-recognizing lectin from marine sponge Craniella australiensis(carter):purification and characterization
作者: Xiong CN(熊川男) ;  Li W(李伟) ;  Zhang W(张卫) ;  Bai XF(白雪芳) ;  Du YG(杜昱光)
会议名称: 13th International Congress on Genes, Gene Families and Isozymes 2005''Forum on Fishery Science and Technology
会议日期: 2005-9-17
出版日期: 2005-09-17
会议地点: 中国
其他题名: 对海绵 Craniella australiensis(carter)中粘蛋白特异性凝集素的分离和鉴定
通讯作者: Liwei
部门归属: 十八室
摘要: From marine sponge Craniella australiensis (Carter) we isolated and characterized a new mucin-recognizing lectin which was purified by a combination of ion-exchange chromatography on DEAE-sephacel and gel filtration. Molecular mass of purified lectin obtained was determined by SDS-PAGE to be 54,000, independent of the presence or absence of 2-mercaptoethanol. It was identified by MALDI-TOF mass spectrometry as a trimer with a subunit molecular mass of 17,800. The lectin was found to agglutinate A, B types of human erythrocytes together with rabbit and sheep. In hemagglutination inhibition assays, asialo-mucin was the best inhibitor. The hemagglutinating activity of the lectin was independent of the divalent cation Ca2+. Significant lectin activity was observed between pH 5-8. The lectin is not sensitive to temperature. The N-terminal amino acid of lectin is Thr.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/111844
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Xiong CN,Li W,Zhang W,et al. Mucin-recognizing lectin from marine sponge Craniella australiensis(carter):purification and characterization[C]. 见:13th International Congress on Genes, Gene Families and Isozymes 2005''Forum on Fishery Science and Technology. 中国. 2005-9-17.
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