中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: 蜗牛酶中水解人参皂甙Rb1的β-D-葡萄糖苷酶的分离纯化及其性质研究
作者: 栾宏伟 ;  齐小辉 ;  杨凌*
会议名称: the 2 international Science and Technology Conference of Traditional Chinese Medicine Modernization
会议日期: 2005-9-24
出版日期: 2005-09-24
会议地点: 中国
其他题名: 蜗牛酶中水解人参皂甙Rb1的β-D-葡萄糖苷酶的分离纯化及其性质研究
通讯作者: 杨凌
ISSN: 7-5364-5832-0
部门归属: 十八室
摘要: Abstract: A β-D-glucosidase from the China white jade snail enzyme had been purified to apparent homogeneity using a combination of anion-exchange, hydrophobic-interaction and gel-permeation chromatography. The enzyme hydrolyzed the 20-C, β-(1→6)-glucoside of ginsenoside Rb1(G-Rb1) to ginsenoside Rd (G-Rd), but did not hydrolyze the other β-D-glucosidic bonds of G-Rb1. This purified glucosidase consisted of two same subunits and had Mr of 230kDa as determined by SDS-PAGE and gel-permeation chromatography. Maximal β-D-glucosidase activity occurred at 70℃ and pH5.6. At pH5.6 and 50℃ the Km for p-nitrophenyl-β-D-glucopyranoside (pNPG) was 0.338mM and it had a Vmax of 0.25mmol nitrophenol/min/mg glucosidase. Theβ-D-glucosidase exhibited low levels of activity against other artificial substrates, compared to its activity against pNPG.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/111858
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
栾宏伟,齐小辉,杨凌*. 蜗牛酶中水解人参皂甙Rb1的β-D-葡萄糖苷酶的分离纯化及其性质研究[C]. 见:the 2 international Science and Technology Conference of Traditional Chinese Medicine Modernization. 中国. 2005-9-24.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [栾宏伟]'s Articles
 [齐小辉]'s Articles
 [杨凌*]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [栾宏伟]‘s Articles
 [齐小辉]‘s Articles
 [杨凌*]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace