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学科主题: 物理化学
题名: Isolation and Purification of a Novel β-Glucosidase Hydrolyzing Ginsenoside-Rb3 to Mx
作者: Hu Y(胡莹) ;  Wang ZY(王卓远) ;  Yang L(杨凌)
会议名称: the 2 international Science and Technology Conference of Traditional Chinese Medicine Modernization
会议日期: 2005-9-24
出版日期: 2005-09-24
会议地点: 中国
其他题名: 一种从人参皂苷Rb3制备Mx的葡萄糖苷酶的分离和纯化
通讯作者: 杨凌
ISSN: 7-5364-5832-0
部门归属: 十八室
摘要: Abstract:Ginsenoside Rb3, one of protopanaxadiol-type ginsenoside, can be hydrolyzed into Mx, one less - polar ginsenoside by SE. Meanwhile, Mx could be a candidate for exploiting an anti-tumor drug. A ginsenoside-Rb3 hydrolyzingβ-glucosidase was purified from SE through following steps, a)DEAE Sepharose CL-6B ion exchange chromatography (IEC), b) TOYOPEARL Butyl-650C hydrophobic interact chromatography (HIC), c) Sephadex G-200 gel filtration chromatography (GFC).The purified emzyme showed a single band on SDS polyacrylamide gel electrophoresis. The molecular weight was estimated by SDS-PAGE and by gel filtration as 58KD and 59KD,respectivly.The optimal reaction conditions for the enzyme were pH 4.8 at 60℃.Among all tested substrates ,the enzyme exhibited the specific activity to p-nitrophenyl β-D-glucopyranoside(pNPG), also poor hydrolyzing activity to p-nitrophenyl-β-D-cellobioside and p-nitrophenyl α-D-xylopyranoside.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/111860
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Hu Y,Wang ZY,Yang L. Isolation and Purification of a Novel β-Glucosidase Hydrolyzing Ginsenoside-Rb3 to Mx[C]. 见:the 2 international Science and Technology Conference of Traditional Chinese Medicine Modernization. 中国. 2005-9-24.
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