DICP OpenIR
学科主题物理化学
Medium Optimization for the Production of Human Cytochrome P450 2C9 by Escherichia Coli BL21(DE3)pLysS using Plackett Burman Design and Grey Relational Analysis
Hao DC(郝大程); Zhu PH(朱盼鸿); Yang SL(杨胜利); Yang L(杨凌)
会议名称The 1st asia Pacific ISSX Meeting
会议日期2006-5-24
2006-05-24
会议地点韩国
页码230/1
部门归属十八室
英文摘要Objective: To optimize the culture medium of the recombinant E. coli, to determine the influence order of 11 factors, and to improve the yield of heterologously expressed CYP2C9. Methods: Plackett-Burman (PB) design, a statistical methodology, was used to screen 11 nutrients and medium components for the production of human CYP2C9 from E. coli BL21(DE3)pLysS harboring plasmid pCW2C9dH in aerobic shaking flask cultures. The experimental data were subject to statistical analysis for calculating the regression coefficients. The table of PB design was also used to construct the series that is needed for grey relational analysis (GRA), an approach totally different from traditional statistical analysis used to calculate the relational grade between two sequence data, to determine the influence priority of 11 parameters. Results: Influence priority: glycerol >δ-ALA > IPTG = ampicillin > chloramphenicol = inoculum density > peptone > thiamine > trace element > NH4Cl > MgSO4. The coefficient for the individual factor estimated from PB design approves the conclusion of GRA. Conclusion: This is the first report that combined two powerful analysis methods for the optimization study of recombinant protein production. (Supported by the 973 program 2003CB716006 of the Ministry of Science & Technology of China, and the Leading Program of the Chinese Academy of Sciences KGCXZ-SW-213-04)
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/112184
专题中国科学院大连化学物理研究所
通讯作者Yang L(杨凌)
推荐引用方式
GB/T 7714
Hao DC,Zhu PH,Yang SL,et al. Medium Optimization for the Production of Human Cytochrome P450 2C9 by Escherichia Coli BL21(DE3)pLysS using Plackett Burman Design and Grey Relational Analysis[C],2006:230/1.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[郝大程]的文章
[朱盼鸿]的文章
[杨胜利]的文章
百度学术
百度学术中相似的文章
[郝大程]的文章
[朱盼鸿]的文章
[杨胜利]的文章
必应学术
必应学术中相似的文章
[郝大程]的文章
[朱盼鸿]的文章
[杨胜利]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。