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学科主题: 物理化学
题名: Medium Optimization for the Production of Human Cytochrome P450 2C9 by Escherichia Coli BL21(DE3)pLysS using Plackett Burman Design and Grey Relational Analysis
作者: Hao DC(郝大程) ;  Zhu PH(朱盼鸿) ;  Yang SL(杨胜利) ;  Yang L(杨凌)
会议名称: The 1st asia Pacific ISSX Meeting
会议日期: 2006-5-24
出版日期: 2006-05-24
会议地点: 韩国
通讯作者: 杨凌
部门归属: 十八室
摘要: Objective: To optimize the culture medium of the recombinant E. coli, to determine the influence order of 11 factors, and to improve the yield of heterologously expressed CYP2C9. Methods: Plackett-Burman (PB) design, a statistical methodology, was used to screen 11 nutrients and medium components for the production of human CYP2C9 from E. coli BL21(DE3)pLysS harboring plasmid pCW2C9dH in aerobic shaking flask cultures. The experimental data were subject to statistical analysis for calculating the regression coefficients. The table of PB design was also used to construct the series that is needed for grey relational analysis (GRA), an approach totally different from traditional statistical analysis used to calculate the relational grade between two sequence data, to determine the influence priority of 11 parameters. Results: Influence priority: glycerol >δ-ALA > IPTG = ampicillin > chloramphenicol = inoculum density > peptone > thiamine > trace element > NH4Cl > MgSO4. The coefficient for the individual factor estimated from PB design approves the conclusion of GRA. Conclusion: This is the first report that combined two powerful analysis methods for the optimization study of recombinant protein production. (Supported by the 973 program 2003CB716006 of the Ministry of Science & Technology of China, and the Leading Program of the Chinese Academy of Sciences KGCXZ-SW-213-04)
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/112184
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Hao DC,Zhu PH,Yang SL,et al. Medium Optimization for the Production of Human Cytochrome P450 2C9 by Escherichia Coli BL21(DE3)pLysS using Plackett Burman Design and Grey Relational Analysis[C]. 见:The 1st asia Pacific ISSX Meeting. 韩国. 2006-5-24.
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