Subject Area物理化学
Potential of marine sponge Hymeniacidon perleve as a bioremediator of pathogenic bacteria in integrated aquaculture ecosystem
Zhang W(张卫); Fu WT(付晚涛); Sun LM(孙黎明); Zhang XC(张喜昌)
Conference Name7thInternational Sponge Symposium 2006-Biotechnology and Pharmacology
Conference Date2006-5-7
Conference Place巴西
Alternative Title繁茂膜海绵生物修复整合养殖生态体系病原微生物的研究
Funding Organization巴西
AbstractThe recurrent incidences of aquaculture diseases world-wide have resulted in huge economic losses for aquaculture industry. One of the key triggering factors is the influx for bacterial pathogens form human and animal wastes through waterways .Sponges (Porifera ) ,as sessile filter –feeding animals ,have extremely high capability to filter the water column .The aim of this work is therefore to investigate the potential of using sponges as a bioremediator to remove pathogen bacteria in integrated aquaculture ecosystem .Using an interidal marine sponge Hymeniacidon perleve as a model system , the ability of removing the most common pathogens E.coil and Vibrio anguillarum II in aquaculture waters were conducted in laboratory tests. In sterilized natural seawater (SNSW)supplemented with E.coil at 7.0-8.3ⅹ106 cells.ml-1,H.perleve can remove an average 96% of E.coil within 10.5 h at a filter rate of ca. 7.53-8.03ⅹ107cells h-1.g-1 of fresh sponge in two independent tests. Despite the removal efficiency and filter rate are similar , the clearance rates (the volume of water cleared of bacteria per unit time and sponge weight )vary significantly among individual sponge specimens and between two batches. For the tests of V.anguillarm II in SNSW ,about 1.5g fresh sponges can keep the pathogen growth under control at a lower initial density 3.6ⅹ106 cells.ml-1of 200ml water volume .Further tests were done for 24 h using about 12 g fresh sponge in 2-L actual seawater collected form two aquaculture sites that have ca .8-fold difference in pathogen bacteria load .The concentrztions of E.coil, Vibrio and total bacteria at 24 h in treatment groups were markedly lower, at about 0.9%,6.2-34.5%and 13.7-22.5% respectively of those in the control. Using a fluoresce stain 1,1`-dioctadecyl-3,3,3`,3`-tetramethylindocarbocyanine perchlorate , E.coil, and Vibrio anguillarum II cells were syained and fed to sponges.The confocalmicroscope observationconfirmed that the sponges filtering –retained and digested these bacteria phagocytosis Potential may well exist for sponges as a bioremediator of bacterial pathogens in integrated aquaculture.
Document Type会议论文
Corresponding AuthorZhang W(张卫)
Recommended Citation
GB/T 7714
Zhang W,Fu WT,Sun LM,et al. Potential of marine sponge Hymeniacidon perleve as a bioremediator of pathogenic bacteria in integrated aquaculture ecosystem[C],2006:44/1.
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