中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: Potential of marine sponge Hymeniacidon perleve as a bioremediator of pathogenic bacteria in integrated aquaculture ecosystem
作者: Zhang W(张卫) ;  Fu WT(付晚涛) ;  Sun LM(孙黎明) ;  Zhang XC(张喜昌)
会议名称: 7thInternational Sponge Symposium 2006-Biotechnology and Pharmacology
会议日期: 2006-5-7
出版日期: 2006-05-07
会议地点: 巴西
其他题名: 繁茂膜海绵生物修复整合养殖生态体系病原微生物的研究
通讯作者: 张卫
部门归属: 十八室
主办者: 巴西
摘要: The recurrent incidences of aquaculture diseases world-wide have resulted in huge economic losses for aquaculture industry. One of the key triggering factors is the influx for bacterial pathogens form human and animal wastes through waterways .Sponges (Porifera ) ,as sessile filter –feeding animals ,have extremely high capability to filter the water column .The aim of this work is therefore to investigate the potential of using sponges as a bioremediator to remove pathogen bacteria in integrated aquaculture ecosystem .Using an interidal marine sponge Hymeniacidon perleve as a model system , the ability of removing the most common pathogens E.coil and Vibrio anguillarum II in aquaculture waters were conducted in laboratory tests. In sterilized natural seawater (SNSW)supplemented with E.coil at 7.0-8.3ⅹ106 cells.ml-1,H.perleve can remove an average 96% of E.coil within 10.5 h at a filter rate of ca. 7.53-8.03ⅹ107cells h-1.g-1 of fresh sponge in two independent tests. Despite the removal efficiency and filter rate are similar , the clearance rates (the volume of water cleared of bacteria per unit time and sponge weight )vary significantly among individual sponge specimens and between two batches. For the tests of V.anguillarm II in SNSW ,about 1.5g fresh sponges can keep the pathogen growth under control at a lower initial density 3.6ⅹ106 cells.ml-1of 200ml water volume .Further tests were done for 24 h using about 12 g fresh sponge in 2-L actual seawater collected form two aquaculture sites that have ca .8-fold difference in pathogen bacteria load .The concentrztions of E.coil, Vibrio and total bacteria at 24 h in treatment groups were markedly lower, at about 0.9%,6.2-34.5%and 13.7-22.5% respectively of those in the control. Using a fluoresce stain 1,1`-dioctadecyl-3,3,3`,3`-tetramethylindocarbocyanine perchlorate , E.coil, and Vibrio anguillarum II cells were syained and fed to sponges.The confocalmicroscope observationconfirmed that the sponges filtering –retained and digested these bacteria phagocytosis Potential may well exist for sponges as a bioremediator of bacterial pathogens in integrated aquaculture.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/112308
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Zhang W,Fu WT,Sun LM,et al. Potential of marine sponge Hymeniacidon perleve as a bioremediator of pathogenic bacteria in integrated aquaculture ecosystem[C]. 见:7thInternational Sponge Symposium 2006-Biotechnology and Pharmacology. 巴西. 2006-5-7.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [张卫]'s Articles
 [付晚涛]'s Articles
 [孙黎明]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [张卫]‘s Articles
 [付晚涛]‘s Articles
 [孙黎明]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace