Subject Area物理化学
Advances in the development of in vitro cell cultures of sponge archaeocytes for the production of sponge-derived bioactive metabolites
Zhang W(张卫); Sun LM(孙黎明); Song YF(宋悦凡); Qu Y(曲翊); Cao XP(曹旭鹏); Jin Y(靳艳)
Conference Name7thInternational Sponge Symposium 2006-Biotechnology and Pharmacology
Conference Date2006-5-7
Conference Place巴西
Alternative Title海绵原细胞体外培养生产海绵来源生物活性代谢产物的研究进展
Funding Organization巴西
AbstractSponges(Porifera)are among the most successful life forms that are the phylogenetically oldest metazoan ,with an estimated 15,000 species alive today .More than 10 percent of sponge spcies investigated have exhibited cytotoxic activity ,which makes them inarguably the best source of novel bioactive compounds with therapeutic potential ,However ,only a few of these compounds have been tested in clinical in trials ,due to the limited availability of naturally occurring sponge materials and the extremely low level of bioactive molecules. This is well known in the clinical development of marine-derived drugs as “pharmaco-apoptosis –programmed drug death ” To succeed in commercial development sponge-based agent will have to be produced in relatively large amounts for detailed development .testing and clinical application .One of the most promising approaches that have been proposed is the application of in vitro culture of sponge primmorphs for the production of bioactive compounds in bioreactors developed by Porf.MÜller,s group. It has been demonstrated that the primmorph culture of Dysidea avara, as a special form of sponge cell aggregates ,can produce the anticancer agent ,avarol. As a further improvement of the primmorph cultures ,our laboratory has developed a new method to establish in vitro primmorph culture form archaeocyte-dominant cell population (ADCP)enriched by Ficoll gradient ,rather than a mixed cell population(MCP).Our rationale is based upon the totipotency of archaeocyte cells and the different biological functions of various sponge cell types .A sponge hymeniacidon pereve collected form China Yellow Sea was used as a model system for the investigation of cell proliferation. In a preliminary enrichment of up to 20% archaeocyte –population, distinct dynamics of primmorph formation were observed while significant increases in DNA synthesis ,cell proliferation (up to 3-fold )and cell growth(up to 4-fold )were achieved. These results demonstrated the importance of archaeocyte and archaeocyte-like cell in successful establishment of in vitro sponge primmorph culture system .Our recent developments have been focusing on the development of advanced purification and fraction techniques to purify archaeocyte cells .An integrated 4-step process was developed while can purify archaeocyte cells up to 90% purity. The in vitro culture of sponge archaecyte cells in various purities has clearly suggested that archaeocyte cells are possibly the proliferating cells among various sponge cell types. As a demonstration , the culture of archaeocyte cells of H.perleve has the ability to grow and biosynthesize the biosynthesize the bioactive metabolites ,however the differentiation is still a problem .To develop a continuous cell lines, factors controlling cell differentiation and promoting cell division will have to determined.
Document Type会议论文
Corresponding AuthorZhang W(张卫)
Recommended Citation
GB/T 7714
Zhang W,Sun LM,Song YF,et al. Advances in the development of in vitro cell cultures of sponge archaeocytes for the production of sponge-derived bioactive metabolites[C],2006:45/1.
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