Subject Area物理化学
Marine sponge Hymeniacidon perleve possesses high diversity culturable actinobacteria : isolation and phylogenetic dieversity by 16S rDNA gene-RFLP analysis
Zhang W(张卫); Zhang HT(张海涛); YooKyungLee; HongKumLee
Conference Name7thInternational Sponge Symposium 2006-Biotechnology and Pharmacology
Conference Date2006-5-7
Conference Place巴西
Alternative Title繁茂膜海绵具有高度多样性可培养的放线菌:16S rDNA-RFLP 分析系统多样性及分离
Funding Organization巴西
AbstractThe class Acinobacteria is a very important group of bacteria that has considerable value as a prolific producer of antibiotics and other therapeutic compounds .With the intense exploitation of terrestrial actinobacteria over many years ,the discovery rate of novel bioactive compounds has fallen steadily .with an estimated 95% rediscovery rate of known compounds. To solve this problem, the isolation of novel actinobacteria form marine environments has been a fruitful area of research in the past decade. However ,little is known about the diversity of actinobacteria form marine species compared to the diverse range of actinobacteria isolated form terrestrial environments .To better understand the actinobacteria diversity with marine sponges. This study attempted to isolate and cultivate actinobacteria form the intertidal marine sponge Hymeniacidon perleve for further physiological studies and to discover bioactive natural products .H.perleve is widely distributed along the coast of the Yellow Sea near Dalian ,China .We recently isolated many antibacterial and antitumor compounds form this sponge. A culture –independent molecular approach has indicated high diversity of the associated bacteria , although no actinobacteria were identified. We wanted to discover whether the sponge harbors actinobacteria that were not detected because of a possible low abundance of Gram-positive actinobacteria .In this study ,a total of 244 actinobacteria associated with marine sponge Hymeniacidon perleve collected form the Yellow Sea ,China were isolated using 13 different media .The number of species and genera actinobacteria recovered form the different media varied significantly ,underlining the importance of optimizing the isolation conditions. The phylogenetic diversity of the actinobacteria isolates was assessed using 16SrDNA amplification-restriction fragment length polymorphism (RFLP) analysis of 106 selected strains with different morphologies. The RPLF fingerprinting of selected strains by HhaI-digestion of 16SrDNA genes resulted in 11 different patterns. The HhaI-RFLP analysis gave good resolution for identifying the actinobacteria isolates at the genus level. A phylogenetic analysis using 16SrDNA sequences revealed a high diversity of seven of culturable actinobacteria including Streptomyces,Nocardiopsis ,Pseudonocardia ,Nocardia ,Micromonospora ,Rhodococcus,and Actinoalloteichus .The dominant genus was Streptomyces ,which represented 73.6% of the isolates. There strains were candidates for new species .
Document Type会议论文
Corresponding AuthorZhang W(张卫)
Recommended Citation
GB/T 7714
Zhang W,Zhang HT,YooKyungLee,et al. Marine sponge Hymeniacidon perleve possesses high diversity culturable actinobacteria : isolation and phylogenetic dieversity by 16S rDNA gene-RFLP analysis[C],2006:46/1.
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