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学科主题: 物理化学
题名: LC–MS/MS identification and quantification of bioactive components in rat serum after orally administrating the decoction of Xuefuzhuyu
作者: Li XL(李秀玲) ;  Liang XM(梁鑫淼)
会议名称: Dalian International Symposia and Exhibition on Chromatography
会议日期: 2007-6-4
出版日期: 2007-06-04
会议地点: 中国
通讯作者: 梁鑫淼
ISSN: 978-7-122-00450-5
部门归属: 十八室
主办者: 中国科学院大连化学物理研究所
摘要: Traditional Chinese medicines (TCMs) attract increasing global attention because of their substantial historical records of efficacy in human beings. However, the relationship between clinical effects and biologically active components of TCM is still unclear. To resolve this problem, it is important to perform component analysis to define actually absorbed components when complex mixtures are given orally. Xuefuzhuyutang (XFZY) is a traditional Chinese formula used to treat arteriosclerosis. Our pharmacological results in rats indicated that XFZY could notably reduce the lipoprotein, low-density lipoprotein and triglyceride levels. To identify and quantify biologically active components in rat serum after orally administrating the decoction of XFZY, LC–MS/MS method was developed and validated. The HPLC separation was carried out on a WatersNovaPakC18 column using acetonitrile and water as mobile phase after the sample of rat serum was cleaned up with solid-phase extraction. Atmospheric pressure chemical ionization in the negative ion mode and selected reaction monitoring (SRM) method were developed to determine the active components. Eight components, gallic acid, caffeic acid, ferulic acid, peaoniflorin, senkyunolide H, hesperidin and neohesperidine, were identified in the serum by comparing their retention times and their independent SRM precursor/product ion transitions with those of corresponding reference standards. The concentrations of hesperidin and neohesperidin in rat serum were quantified by SRM measurement, which were 12.5and16.1 ng/ml. This method was validated in terms of recovery, linearity, accuracy and precision (intra- and interday variation).The recoveries from spiked control samples were 93.0 and 89.3 for hesperidin and neohesperidin. Linearity in rat serum was observed over the range of 2.0–50.0ng/ml. Percent bias (accuracy) and precision were well within the acceptable range and the relative standard deviation (R.S.D.) of the measured rat serum samples was less than10% (n = 5). A highly selective and sensitive LC–MS/MS method was developed and validated for the simultaneous qualification and quantification of bioactive components in rat serum after oral administration of TCM. The method is useful for screening potential drugs at low levels in biological fluids and highly suitable for carrying out preclinical or pharmacokinetic studies of TCMs.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/112472
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Li XL,Liang XM. LC–MS/MS identification and quantification of bioactive components in rat serum after orally administrating the decoction of Xuefuzhuyu[C]. 见:Dalian International Symposia and Exhibition on Chromatography. 中国. 2007-6-4.
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