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学科主题物理化学
Analysis of strong polar compounds in extract of Gardenia jasminoisdes fruit using hydrophilic interaction liquid chromatography with evaporative light-scattering detection
Ren LL(任玲玲); Xue XY(薛兴亚); Zhang FF(章飞芳); Xu Q(徐青); Liang XM(梁鑫淼)
会议名称Dalian International Symposia and Exhibition on Chromatography
会议日期2007-6-4
2007-06-04
会议地点中国
页码14/2
部门归属十八室
主办者中国科学院大连化学物理研究所
英文摘要The hydrophilic interaction liquid chromatography (HILIC) is an alternative HPLC mode for separation of polar compounds with polar stationary phases like NP-HPLC but aqueous mobile phase similar to RP-HPLC mode. Evaporative light-scattering detection (ELSD) is an important detector when UV detection is not feasible. The ELSD has been shown to successfully detect many substances such as phospholipids, triglycerides, fats and fatty acid esters, carbohydrates, synthetic polymers, steroids, amino acids and inorganic ions, such as chloride and sodium cations. In this study the fraction eluted at 2-3min in RP-HPLC of crude extract of Gardenia jasminoisdes fruit was collected and separated by the combination of HILIC with ELSD through study the effect of formic acid and the content of formic acid in mobile phase. The mobile phase was prepared using two solutions, A and B, where A was 0.5% (V/V) formic acid in water, and B was 0.5% (V/V) formic acid in acetonitrile solution. The analytes were eluted from the column with a linear gradient of 10% A to 40% A over 30 minutes and holding 40%A for 5 minutes. The chromatography of strong polar compounds of fruit of Gardenia jasminoisdes is shown in Fig 1. The precision of retention time of the method was determined by injecting six times. Except peak 2 all the RSD were less than 0.13% and the RSD of peak 2 was 0.22%. The retention mechanism of peak 1 and 3 was also studied. The result indicated that the mechanisms of the two peaks were HILIC mode and it can be regressed with the equation: lnk’=a+blnCB+cCB with an average R2 of 0.9999 for peak1 and 0.9953 for peak 3.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/112486
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼)
推荐引用方式
GB/T 7714
Ren LL,Xue XY,Zhang FF,et al. Analysis of strong polar compounds in extract of Gardenia jasminoisdes fruit using hydrophilic interaction liquid chromatography with evaporative light-scattering detection[C],2007:14/2.
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