Subject Area物理化学
Study of Flavonoid Aglycones by High Performance Liquid Chromatography
Wang YP(王艳萍); Xue XY(薛兴亚); Zhang FF(章飞芳); Xu Q(徐青); Liang XM(梁鑫淼)
Conference NameDalian International Symposia and Exhibition on Chromatography
Conference Date2007-6-4
Conference Place中国
Funding Organization中国科学院大连化学物理研究所
AbstractFlavonoids are the largest group of secondary metabolites in plants. The flavonoid family comprises 15 classes of compounds. Their detection and identification have been extensively investigated in the past and during recent years, specifically because of their biological and physiological importance. In our present work, we studied the structure-retention relationship of the flavonoid aglycones, which can perform an assistant role in the MS characterization. HPLC and CSASS software [1] were performed to obtain the retention parameters a and c (a, c are constants and related to the structure in a given chromatographic system). We found there were some relationships between structure and retention parameters. In this study, five sub-classes of flavonoid aglycones---flavones, isoflavones, flavonols, flavanones and isoflavonones---were included; and two kinds of substituents---hydroxyl and methoxyl---were covered. The relationships between a and -c are shown in figure 1. It is easy to see from figure 1, either the basic structure of the aglycones or the kind of the substituents has great relationship with their retention behavior. The influence induced by the hydroxyl in B-ring is especially observable. The compounds are divided into two separated categories based on the hydroxyl in B-ring. Those without hydroxyl in B-ring have strong retention on the RP-HPLC, and their retention parameters are in area Ⅰ. Contrarily, while there is one or more hydroxyl in B-ring, their retention parameters are in area Ⅱ. Besides, the a and c values of different sub-classes of flavonoid aglycones are in different areas, while there are hydroxyls in B-ring. And in the same sub-class, the number or the position of hydroxyl substituents also have great influence on their retention behavior. In conclusion, there are specifically relationships between the structure of compounds and their retention behavior in a given chromatographic system.
Document Type会议论文
Corresponding AuthorLiang XM(梁鑫淼)
Recommended Citation
GB/T 7714
Wang YP,Xue XY,Zhang FF,et al. Study of Flavonoid Aglycones by High Performance Liquid Chromatography[C],2007:21/2.
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