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学科主题: 物理化学
题名: Development and application of sol-gel immunoaffinity extraction-based methods for the determination of diuron in surface water
作者: Zhang XL(张秀莉) ;  DieterMartens ;  PetraM.Kr&auml ;  mer ;  AntoniusA.Kettrup ;  Liang XM(梁鑫淼)
会议名称: Dalian International Symposia and Exhibition on Chromatography
会议日期: 2007-6-4
出版日期: 2007-06-04
会议地点: 中国
通讯作者: 梁鑫淼
部门归属: 十八室
主办者: 中国科学院大连化学物理研究所
摘要: An anti-diuron immunoaffinity extraction column was fabricated for the first time by encapsulation of anti-diuron monoclonal antibodies in sol-gel glass matrix. The experimental capacity of the sol-gel immunoaffinity column (SG-IAC) immobilized 240 g antibodies was 302.1±11.6 ng (n=4) of diuron, corresponding to 40% of the theoretical maximum capacity. The antibody density in SG-IAC up to 3.75 mg g-1 resulted the linear increasing of experimental capacity. The immunoaffinity column-based loading, rinsing and eluting processes were optimized. Based on these optimizations, the SG-IAC selectively extracted diuron from an artificial mixture of 64 pesticides. In addition to this high selectivity, the SG-IAC proved to be reusable. The SG-IAC was combined with liquid chromatography-tandem mass spectrometry to determine diuron in surface water, and the linear range was up to 2.2 g L-1 with correlation coefficient higher than 0.99 and relative standard deviation lower than 5.7% (n=8). The limit of quantitation for 25-mL surface water sample was 3.9 ng L-1.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/112500
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhang XL,DieterMartens,PetraM.Krä,et al. Development and application of sol-gel immunoaffinity extraction-based methods for the determination of diuron in surface water[C]. 见:Dalian International Symposia and Exhibition on Chromatography. 中国. 2007-6-4.
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