Subject Area物理化学
Strategically searching for estrogenic compounds from Radix puerariae
Zhang Y(张岩); Zhang XZ(张晓哲); Zhang FF(章飞芳); Xue XY(薛兴亚); Xu Q(徐青); Liang XM(梁鑫淼)
Conference NameDalian International Symposia and Exhibition on Chromatography
Conference Date2007-6-4
Conference Place中国
Funding Organization中国科学院大连化学物理研究所
AbstractPhytoestrogens have received increasing interest in recent years as possible candidates for alternative hormone replacement therapy (HRT). In this paper, we report one efficient way to search for estrogenic compounds in Radix puerariae. Preparative HPLC was used as a powerful tool for bio-guided separation of the ethyl acetate (EtOAc) fraction from the crude extract into 29 well-characterized sub-fractions. Fractionation was followed by the combination of bioactive screening by recombinant yeast estrogen screening (YES) assay and chemical screening by HPLC and/or HPLC-MS. EC50 values obtained from YES showed that 10 sub-fractions of F17 to F26 have pronounced estrogenic activities in 103~106 fold less than that response of 17β-estradiol (E2). Both biological data and chemical data were correlated. From E2 equivalent (EEQ) values, two sub-fractions, F24 and F25, were observed to contribute most to the estrogenic activity of the EtOAc fraction. Four compounds were identified to contribute to the high estrogenic activity in Radix puerariae, daidzein, genistein, formononetin and coumestrol, which did not have various isolation and purification steps involved in the traditional approach for bio-guided fractionation. Although no novel biological compounds with interesting chemical or spectroscopic features were found, a strategic search method for potential bioactive compounds is presented, which facilitates optimization of an investigation and tedious fractionation of the sample matrices is circumvented.
Document Type会议论文
Corresponding AuthorLiang XM(梁鑫淼)
Recommended Citation
GB/T 7714
Zhang Y,Zhang XZ,Zhang FF,et al. Strategically searching for estrogenic compounds from Radix puerariae[C],2007:26/2.
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