Subject Area物理化学
Method development and applications of comprehensive two-dimensional gas chromatography
Lu X(路鑫); Kong HW(孔宏伟); Ye F(叶芬); Pang T(逄涛); Li X(李响); Qiu YQ(邱涯琼); Xu GW(许国旺)
Conference NameDalian International Symposia and Exhibition on Chromatography
Conference Date2007-6-4
Conference Place中国
Funding OrganizationCAS等
AbstractComprehensive two-dimensional gas chromatography (GC×GC) is a high resolution and high sensitivity technique. It is especially suitable for the separation and identification of analytes in complex mixtures. Focused on the characterization of complex mixtures, a series of method and application studies using GC×GC have been carried out. Although comprehensive two-dimensional gas chromatography (GC×GC) can offer high resolution and sensitivity compared with traditional one-dimensional gas chromatography (1DGC), sample preparation is still essential for trace component analysis. An on-column cold trap device was used as a part of GC×GC or GC×GC combined with time-of-flight mass spectrometry (TOFMS) systems. The on-column cold trap device using a homemade double layer CO2 jet was mounted on the GC×GC or GC×GC-TOFMS, and performed at -60 ℃-70 cryogenic ℃temperature. Based on large volume injection, the trace compounds were focused at the head of column by cold trap and stationary phase, and thermally desorbed and reinjected by oven. The performance of on-column cold trap was evaluated. Due to no extra increment of system dead volume by the on-line enrichment, the bandwidths of on-column cold trap were equal to those of directly injection. Components whose boiling points were no less than that of nonane could be captured entirely by this method. The system was applied to trapping trace components in gas, liquid or solid matrix. The detection sensitivity was improved remarkably using these systems. The method greatly reduced sample preparation time and sample volume compared with off-line sample extraction methods. The results indicate that this on-column cold-trap combined with GC×GC system is very useful for detection and determination of trace compounds in complex mixture. A GC×GC-TOFMS in combination with multivariate analysis method were developed for characterization complex mixtures, such as traditional Chinese medicine (TCM) and tobacco. The method was used to classify TCM from different regions, and identify the potential marker compounds that lead to the differentiation. Different types of tobacco and cigarette were also evaluated using this method. A GC×GC-TOFMS based metabonomics analysis method were developed. The bottleneck problems of “omics” studies using GC×GC-TOFMS based technique were indicated and solved step by step. Algorithms of peak alignment and peak matching have been developed before the pattern recognition. A method used for accurate identification of potential biomarker was developed. Some examples of application were given.
Document Type会议论文
Corresponding AuthorXu GW(许国旺)
Recommended Citation
GB/T 7714
Lu X,Kong HW,Ye F,et al. Method development and applications of comprehensive two-dimensional gas chromatography[C],2007:18/1.
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