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学科主题物理化学
Simultaneous separation of hydrophilic and hydrophobic components with single injection by column-switching HPLC
Wang Y(王媛); Lu X(路鑫); Xu GW(许国旺)
会议名称Dalian International Symposia and Exhibition on Chromatography
会议日期2007-6-4
2007-06-04
会议地点中国
页码213/2
ISSN978-7-122-00450-5
部门归属十八室
主办者CAS等
英文摘要Column-switching HPLC has been widely used in the last several decades. When the target analytes are retained on the both columns, it is named “two-dimensional (2D)” HPLC. If only one or several interested analytes were extracted from the matrix by the first dimensional pre-column and separated on the second dimensional analytical column, it is named “heart-cutting” 2D HPLC. When each component of the sample is separated on the both dimensional columns with different retention mechanisms, it is the “comprehensive” 2D HPLC. The comprehensive 2D HPLC technique is suitable for the separation of entire-component in the complex samples, for which the peak capacity of the single-column is limited. Over a few thousand of peak capacity is expected by adding the separation capability of MS detection. Commercial available HPLC modules are easily modified to a comprehensive 2D HPLC system, but the separation time is ultra long by the limitation of the hardware. In addition, the data treatment process of the comprehensive 2D HPLC is inconvenient with additional software. We developed a novel column-switching HPLC technique which is also aimed for the entire-component separation of complex samples but based on the different integration and operation protocols from the comprehensive 2D HPLC. The sample is injected on a HILIC column, in which all the components of the sample are divided into two fractions. The hydrophobic components are eluted at the dead time and transferred into a RP column to fulfill their separation. Meanwhile the hydrophilic ones are separated on the HILIC column. This technique is not operated in any 2D mode, since each component is only separated on one column. Its theoretical peak capacity is about the sum of that of the two analytical columns. On the other hand, the main approach of this technique is to obtain the information of both hydrophilic and hydrophobic components with one injection. Secondly, the throughput of this technique is much higher than that of the comprehensive 2D HPLC. The whole separation time is near to that in a single-column separation process. Thirdly, it is flexible in operation according to different separation purposes. The separation results of the two analytical columns can be recorded in two individual chromatograms simultaneously by two detectors, or, in turn recorded with one detector by trimming of the flow path. This makes it feasible to use MS as the detector to obtain more sample information. Fourthly, the data treatment process is just as same as that in single-columns separation with commercial workstations. No additional software is needed. The repeatability of the column-switching HPLC system is good enough to perform qualification and quantitation. In brief, this novel column-switching HPLC technique can be applied for the analysis of entire components in a complex sample base on their hydrophobic properties. It is easy operating and suitable to hyphenate with MS. Although its theoretical peak capacity is lower than that of comprehensive 2D HPLC, this technique can be applied as a complementary method for high-speed separation of entire components in the complex sample with one injection.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/112542
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺)
推荐引用方式
GB/T 7714
Wang Y,Lu X,Xu GW. Simultaneous separation of hydrophilic and hydrophobic components with single injection by column-switching HPLC[C],2007:213/2.
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