中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: Simultaneous separation of hydrophilic and hydrophobic components with single injection by column-switching HPLC
作者: Wang Y(王媛) ;  Lu X(路鑫) ;  Xu GW(许国旺)
会议名称: Dalian International Symposia and Exhibition on Chromatography
会议日期: 2007-6-4
出版日期: 2007-06-04
会议地点: 中国
通讯作者: 许国旺
ISSN: 978-7-122-00450-5
部门归属: 十八室
主办者: CAS等
摘要: Column-switching HPLC has been widely used in the last several decades. When the target analytes are retained on the both columns, it is named “two-dimensional (2D)” HPLC. If only one or several interested analytes were extracted from the matrix by the first dimensional pre-column and separated on the second dimensional analytical column, it is named “heart-cutting” 2D HPLC. When each component of the sample is separated on the both dimensional columns with different retention mechanisms, it is the “comprehensive” 2D HPLC. The comprehensive 2D HPLC technique is suitable for the separation of entire-component in the complex samples, for which the peak capacity of the single-column is limited. Over a few thousand of peak capacity is expected by adding the separation capability of MS detection. Commercial available HPLC modules are easily modified to a comprehensive 2D HPLC system, but the separation time is ultra long by the limitation of the hardware. In addition, the data treatment process of the comprehensive 2D HPLC is inconvenient with additional software. We developed a novel column-switching HPLC technique which is also aimed for the entire-component separation of complex samples but based on the different integration and operation protocols from the comprehensive 2D HPLC. The sample is injected on a HILIC column, in which all the components of the sample are divided into two fractions. The hydrophobic components are eluted at the dead time and transferred into a RP column to fulfill their separation. Meanwhile the hydrophilic ones are separated on the HILIC column. This technique is not operated in any 2D mode, since each component is only separated on one column. Its theoretical peak capacity is about the sum of that of the two analytical columns. On the other hand, the main approach of this technique is to obtain the information of both hydrophilic and hydrophobic components with one injection. Secondly, the throughput of this technique is much higher than that of the comprehensive 2D HPLC. The whole separation time is near to that in a single-column separation process. Thirdly, it is flexible in operation according to different separation purposes. The separation results of the two analytical columns can be recorded in two individual chromatograms simultaneously by two detectors, or, in turn recorded with one detector by trimming of the flow path. This makes it feasible to use MS as the detector to obtain more sample information. Fourthly, the data treatment process is just as same as that in single-columns separation with commercial workstations. No additional software is needed. The repeatability of the column-switching HPLC system is good enough to perform qualification and quantitation. In brief, this novel column-switching HPLC technique can be applied for the analysis of entire components in a complex sample base on their hydrophobic properties. It is easy operating and suitable to hyphenate with MS. Although its theoretical peak capacity is lower than that of comprehensive 2D HPLC, this technique can be applied as a complementary method for high-speed separation of entire components in the complex sample with one injection.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/112542
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Wang Y,Lu X,Xu GW. Simultaneous separation of hydrophilic and hydrophobic components with single injection by column-switching HPLC[C]. 见:Dalian International Symposia and Exhibition on Chromatography. 中国. 2007-6-4.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [王媛]'s Articles
 [路鑫]'s Articles
 [许国旺]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [王媛]‘s Articles
 [路鑫]‘s Articles
 [许国旺]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace