DICP OpenIR
学科主题物理化学
Studies on pathway of biocatalysis producting terephthalic acid by co-metabolism
Sang P(桑萍); Tian J(田晶); Gao P(高鹏); Xu GW(许国旺)
会议名称Dalian International Symposia and Exhibition on Chromatography
会议日期2007-6-4
2007-06-04
会议地点中国
页码243/2
ISSN978-7-122-00450-5
部门归属十八室
主办者CAS等
英文摘要Biological processes are commonly perceived as being less harmful to environment than chemical processes. A variety of chemical routes to synthesize terephthalic acid are known. But biological process to produce terephthalic acid is almost not. The pathway of biocatalysis producting terephthalic acid by co-metabolism of Comamonas testosterone strain DSM6577 and Stenotrophomonas maltophilia was studied in this thesis. A detecting method of middle metabolites produced at the process of transforming p-xylene to terephthalic acid by HPLC was established. The Hypersil SAX column ( 250 mm ×416 mm ×5μm) was used . The temperature of column was 30°C and detected wavelength at 254 nm. The mobile phase was 0.25 mol/L NH4H2PO4 containing 10 % acetonitrile. The flow rate was 0.8 ml/ min. Comamonas testosterone strain DSM6577 was shown to be capable of oxidizing p-toluic acid and stenotrophomonas maltophilia was shown to be capable of oxidizing p-xylene as sole souce of carbon into terephthalic acid through sequential reactions catalyzed by a series of enzymes including monooxygenase and dehydrogenase. P-toluic acid, p-xylene’s degradation and erephthalic acid production were studied respectively. The terephthalic acid producing organism comprises the genes encoding enzymes involved in the enzymatic conversion of p-xylene to terephthalic acid as follows: p-xylene was acted by xylene monooygenase to form 4-methylbenzyl alcohol, which was acted by methylbenzyl alcohol dehydrogenase to form p-tolualdehyde. And then, at the action of tolualdehyde dehydrogenase, p-tolualdehyde was transformed to p-toluic acid, which is acted by toluate methyl monooxygenase to form 4-carboxybenzyl alcohol. At last, 4-carboxybenzyl alcohol was acted by carboxybenzyl alcohol methyl dehydrogenase to form 4-carboxybenzaldehyde, which was acted by caboxybenzaldehyde methyl dehydrogenase to form terephthalic acid. This enzymatic pathway is referred to be the biosynthetic pathway of terephthalic acid.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/112550
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺)
推荐引用方式
GB/T 7714
Sang P,Tian J,Gao P,et al. Studies on pathway of biocatalysis producting terephthalic acid by co-metabolism[C],2007:243/2.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[桑萍]的文章
[田晶]的文章
[高鹏]的文章
百度学术
百度学术中相似的文章
[桑萍]的文章
[田晶]的文章
[高鹏]的文章
必应学术
必应学术中相似的文章
[桑萍]的文章
[田晶]的文章
[高鹏]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。