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学科主题: 物理化学
题名: The influence of environment to metabolic pathway of microorganisms based on rp-hplc
作者: Tian J(田晶) ;  Shi CY(史春云) ;  Liu H(刘红) ;  Gao P(高鹏) ;  Sang P(桑萍) ;  Xu GW(许国旺)
会议名称: Dalian International Symposia and Exhibition on Chromatography
会议日期: 2007-6-4
出版日期: 2007-06-04
会议地点: 中国
通讯作者: 许国旺
ISSN: 978-7-122-00450-5
部门归属: 十八室
主办者: CAS等
摘要: Now the researches on alkalophilic microorganisms mainly focus on the culture and enzyme extraction and there is few investigation of metabolic pathway. In this paper, Lp3-3 was selected as the experiment strain. The detecting method of metabolites in cell and fermentation liquid was developed based on RP-HPLC, and the influence of environment to metabolism was studied. A detecting method of organic acids in fermentation liquid of Lp3-3 by RP-HPLC was established. The Sinochrom ODS–BP column (250 mm ×4.6mm, 5μm) was used. Mobile phase was 2.5 mmol/L NH4H2PO4 (1% methanol,pH 2.5). The flow rate was 0.5 mL / min, the temperature of column was 30°C and detected wavelength at 210 nm. The method was used to analyze content of organic acids in fermentation liquid of alkalophilic microorganisms, the diversification trend of lactic acid, acetic acid and pyruvic acid at different fermentation time was obtained. In order to study the metabolic pathway of alkalophilic microorganisms, a detecting method of 17 amino acids in cells of alkalophilic microorganisms by HPLC was studied. The chromatographic Sinochrom ODS–BP column was used. Mobile phase were acetonitrile: water=1:1 (solution A) and 0.06mol·/ L + 0.1% N, N – dimethyl – Formamide + 10% acetonitrile, pH was adjusted to 6.4 with 5% acetum (solution B). The flow rate was 0.8 mL / min, the temperature of column was 35°C and detected wavelength at 360 nm. The method was used to analyze content of amino acids in cells of alkalophilic microorganisms at different ferment time, the diversification trend was obtained, and the interrelated metabolizing pathway was studied. By changing culture conditions, the influence of metabolic pathway of Lp3-3 was analyzed, which help us to know the lactic acid dehydrogenase is the main factor to influence the metabolism of Lp3-3.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/112564
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Tian J,Shi CY,Liu H,et al. The influence of environment to metabolic pathway of microorganisms based on rp-hplc[C]. 见:Dalian International Symposia and Exhibition on Chromatography. 中国. 2007-6-4.
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