中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: Characterization of anthocyanin transport and storage in Vitis vinifera L. cv. Gamay Freaux cell suspension cultures
作者: Zhang W(张卫) ;  SConn ;  CFranco
会议名称: 13th European Congress on Biotechnology
会议日期: 2007-9-16
出版日期: 2007-09-16
会议地点: 西班牙
其他题名: 葡萄悬浮细胞培养花青素的贮存转运特征
通讯作者: 张卫
部门归属: 十八室
主办者: 西班牙
摘要: Anthocyanins are ubiquitous plant pigments with strong antiox¬idant activity, stimulating interest in the development of a plant cell-based bioprocess for their production to replace toxic syn¬thetic food dyes and for application as pharmaceuticals, or nutraceuticals. Anthocyanin-producing plant cell suspension cultures are the currently favoured model production system facilitating rapid scale-up of production and circumventing the seasonal growth of crop plants. However, the level of anthocyanin production in these cells is commonly less than that seen in the intact plant, requiring anthocyanin enhance¬ment strategies to improve the commercial feasibility of this approach. Attempts to enhance anthocyanin production by aug¬menting anthocyanin biosynthesis alone, without considering the post-biosynthetic limitations (transport and storage) have been largely unsuccessful in the development of a commercial bioprocess.Theaimsofthisstudyweretocharacterisetheantho¬cyanin transport pathway and storage sites in VitisviniferaL. suspension cells towards significantly improving anthocyanin production by rational enhancement strategies at the molec¬ular level. Anthocyanins are thought to be transported from their site of biosynthesis in the cytosol via the non-covalent (ligandin) activity of glutathione S-transferases (GSTs) to the vacuole where they are concentrated in insoluble bodies, called anthocyanic vacuolar inclusions (AVIs). Five GSTs were affinity purified from pigmented grape sus¬pension cells, characterised by nano-LC MS/MS and Edman sequencing, with the coding sequences identified and cloned. Bombardment of anthocyanin transport-deficient maize ker¬nels with V.viniferaL. GST sequences indicated the potential involvement of two GSTs, GST1 and GST4, in anthocyanin transport.GeneexpressionanalysesbyQPCRindicatedastrong correlation of these two GSTs with anthocyanin accumulation. GST4 was enhanced 60-fold with ver´ aisoninShirazberryskins, while GST1 and to a lesser extent GST4, was induced in V.viniferaL. cv. Gamay Fr´ eaux suspension cells under elicita¬tion with sucrose, jasmonic acid and light irradiation (S/JA/L) to enhance anthocyanin synthesis. Purified GSTs quantified by reverse-phase HPLC from control and S/JA/L-treated sus¬pension cells supported the gene expression data. Sequence alignments of these genes with known anthocyanin-transporting GSTs have shown conserved putative anthocyanin-binding regions. Furthermore, analysis of short upstream regions identi¬fied anthocyanin transcription factor (R/C1)-binding regions in the promoter of GST1. Increasing the expression of these GSTs provides an avenue to enhance anthocyanin production by more rapid removal of anthocyanins from biosynthetic complexes, potentially increasing biosynthetic flux. AVIshavebeendocumentedin45ofthehighestanthocyanin¬accumulating suspension cell cultures, with few detailed studies on their composition, or anthocyanin profile. AVIs in grape cell cultures were found to be highly dense, membrane-delimited bodies containing a complex mix of anthocyanins, long-chain tanninsandotherunidentifiedorganiccompounds.Furthermore, while the proportion of individual anthocyanin species were maintained between whole-cell and AVI extracts, the AVIs were found to selectively bind a subset of highly stable acylated (p-coumaroylated) anthocyanins. Strategies to enhance antho¬cyanin accumulation in grape suspension cultures lead to a proportionate increase in the abundance of AVIs. Unlike AVIs in sweet potato and, to a lesser extent lisianthus, protein was not a major component of AVIs in V.viniferaL. It is likely from this evidence that AVIs represent a by-product of ER-derived vesicular transport of anthocyanins, and therefore not a target for rational enhancement of anthocyanin production.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/112612
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Zhang W,SConn,CFranco. Characterization of anthocyanin transport and storage in Vitis vinifera L. cv. Gamay Freaux cell suspension cultures[C]. 见:13th European Congress on Biotechnology. 西班牙. 2007-9-16.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [张卫]'s Articles
 [SConn]'s Articles
 [CFranco]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [张卫]‘s Articles
 [SConn]‘s Articles
 [CFranco]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace