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学科主题: 物理化学
题名: Interaction between polymer distribution and viability of the encapsulated cells detected by confocal laser scanning microscope
作者: Lv GJ(吕国军) ;  Wang W(王为) ;  Ma XJ(马小军)
会议名称: The 2nd Asian Symposium on Emulsion Polymerization and Functional Polymeric Microspheres
会议日期: 2007-9-9
出版日期: 2007-09-09
会议地点: 中国
通讯作者: 马小军
部门归属: 十八室
主办者: 中科院北京过程所
摘要: Cell microencapsulation is a promising therapeutic strategy to many diseases, among them, Alginate–poly-L-lysine (PLL) –alginate (APA) microcapsules are the far most studied system for encapsulation of living cells. However, the effect of the inner polymer, alginate, on the cell viability is not very clear due to poor technique available to study the distribution of polymers in alginate–PLL–alginate microcapsules. In addition, alginate is used extensively in tissue engineering applications for its many favorable properties required in biomaterials. In this study, a combination of fluorescence labeled alginate and confocal laser scanning microscope (CLSM) provides the information both about the alginate distribution changes during the process of encapsulation and the interaction between the alginate distribution and the viability of the encapsulated cells. Firstly, fluoresceinamine is chosen as a fluorescent dye to label alginate. The influences of pH value of alginate solution, the temperature, the NaCl concentration and the activation ratio of uronic acid in alginate were optimized to offer good reaction efficiencies. As a result, a quick and stable way to prepare fluorescence labeled alginate was set up. It was found that 5% uronic acid activation condition, lower pH and high NaCl concentration provide significant increasing of reaction efficiencies while not affecting the ionic gelling capacity of the alginate to form Ca-alginate gel beads. Secondly, CLSM was applied as a non-destructive and simple method for studying the distribution of fluorescent-labeled alginate in alginate–PLL–alginate (APA) microcapsules made either by the internal gelation or external gelation. The results revealed that inner of APA microcapsules exhibit a concentration inhomogeneity where the polymer concentration is considerably lower in the center of the gel than at the surface. The polymer concentration at the surface is four times higher than the center part, which indicates that this polymer gradient may be an important factor to influence the cell viability. Finally, in order to study the effect of this inhomogeneity in polymer gradient on the cell viability, the effect of different concentration of alginate on the cell viability were evaluated by the MTT method to mimic the polymer distribution in APA microcapsules. The results showed that the cell growth in lower concentration of alginate medium was superior to that achieved in higher concentration of alginate medium, which indicated that this inhomogeneity might be a preferred structure to maintain high viability of microencapsulated cell and high stability of microcapsules.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/112748
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Lv GJ,Wang W,Ma XJ. Interaction between polymer distribution and viability of the encapsulated cells detected by confocal laser scanning microscope[C]. 见:The 2nd Asian Symposium on Emulsion Polymerization and Functional Polymeric Microspheres. 中国. 2007-9-9.
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