DICP OpenIR
Subject Area物理化学
Degree of deacetylation effects on biodegradation of chitosan-alginate polyelectrolyte complexes
Li XX(李晓霞); Xie HG(谢红国); Ma XJ(马小军); Xiaojun MA
Conference Name2 nd Asian Symposium on Emulsion Polymerization and Functional Polymeric Microspheres
Conference Date2007-9-9
2007-09-09
Conference Place中国
Alternative Title脱乙酰度对壳聚糖-海藻酸钠聚电解质复合物降解的影响
Pages45/1
Department十八室
Funding Organization中科院过程工程研究所
AbstractMicrocapsules made of polyelectrolyte complexes(PECs) have shown potential in the area of cell transplantation, enzyme immobilization and drug delivery system. PECs are formed when oppositely charged polyelectrolytes are mixed and interact via electrostatic interactions. Chitosan, which is a copolymer derived from natural polymer chitin and composed of N-acetyl-D-glucosamine and D-glucosamine and alginate, which is a polysaccharide derived from brown seaweed and comprises blocks of β-D-guluronic and α-L-mannuronic, can be used to form PECs, and then obtain chitosan-alginate complex microcapsules. . The successful application of microcapsules requires an understanding of the biodegradation of materials. Since chitosan is degraded by enzymatic systems in vivo, depending on the degree of deacetylation (DD), it was hypothesized that chitosan-alginate complexes could also be biodegraded. Therefore, the current study focused on PECs of sodium alginate and chitosans with DD of 93%, 81%, 72% and 61%, evaluating their structures by FTIR and XRD and biodegradation behaviors in vitro with lysozyme system. The degradation studies were performed at 37 oC and 120 rpm. The degradation kinetics was assessed by evaluating the lysozyme and cumulative degradation products in solution with Bradford method and potassium ferricyanide method. Meanwhile, the weight-loss of PECs was monitored during the whole degradation process and the morphological structure was observed by SEM. Lysozyme in the solution was adsorbed onto the complexes on the first day, and the lower the DD was, the more lysozyme was adsorbed. Meanwhile, the degradation started and reducing sugars were produced, and the lower the DD was, the more reducing sugars released. After 8 weeks of degradation, PEC from DD61% had the most loss-weight and reducing sugars, but PEC from DD91% was nearly degraded. SEM image also showed that there became fibrillar structures in PEC from DD61%, but PEC from DD93% retained the primary structure. Our results revealed that biodegradation of chitosan-alginate PECs are highly dependent on DD of chitosans, though the result of the FTIR and XRD indicated that DD had little influence on the structure of PECs. It is suggest a better control of the degradation kinetics when chitosan-alginate microcapsules are used in biomedical engineering.
Language中文
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/112752
Collection中国科学院大连化学物理研究所
Corresponding AuthorXiaojun MA
Recommended Citation
GB/T 7714
Li XX,Xie HG,Ma XJ,et al. Degree of deacetylation effects on biodegradation of chitosan-alginate polyelectrolyte complexes[C],2007:45/1.
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