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学科主题物理化学
Molecular Cloning and Characterization of an Oligochitosan induced MAP Kinase from Oilseed
Yin H(尹恒); Zhao XM(赵小明); Du YG(杜昱光)
会议名称2008 Annual Meeting of the Society for Glycobiology
会议日期2007-11-11
2007-11-11
会议地点美国
页码167/1
部门归属十八室
主办者Glycobiology
英文摘要We isolated a new MAPK cDNA, designated BnMPK4, from oilseed (Brassica Napus H) based on our former microarray data. BnMPK4 encodes a 363-amino-acid protein with high nucleotide sequence similarity to previously reported plant MAPK genes such as AtMPK4. Used RT-PCR technique we found that BnMPK4 has a constitutive expression in seedling leaves and is further up-regulated upon wounding by cut, treatment with global signal jasmonic acid (JA), fungal elicitor chitosan and oligochitosan but don’t respond to salicylic acid (SA). A 69kD GST-BnMPK4 fusion protein was purified by using PGEX-4T-1 vector and antiserum was prepared use this fusion protein. Western blot results showed that BnMPK4 is activated by oligochitosan rapidly. These results suggest a role for BnMPK4 in JA-regulated defense signalling pathway.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/112838
专题中国科学院大连化学物理研究所
通讯作者Du YG(杜昱光)
推荐引用方式
GB/T 7714
Yin H,Zhao XM,Du YG. Molecular Cloning and Characterization of an Oligochitosan induced MAP Kinase from Oilseed[C],2007:167/1.
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