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学科主题: 物理化学
题名: Characterization of lectin from Helianthus tuberosus L. and effects of crude extract proteins on rabbit-isolated ileum
作者: Xiong CN(熊川男) ;  Zhao XM(赵小明) ;  Yin H(尹恒) ;  Du YG(杜昱光)
会议名称: 2007 Annual Meeting of the Society for Glycobiology
会议日期: 2007-11-11
出版日期: 2007-11-11
会议地点: 美国
通讯作者: 杜昱光
部门归属: 十八室
主办者: glycobiology
摘要: A lectin from Helianthus tuberosus L. was purified and its effects of crude extract proteins on rabbit-isolated ileum was investigated.DEAD-Sephacel and Sephadex G-200 were used to purified the lectin; SDS-PAGE and MALDI-TOF were used to obtain the molecular mass of the lectin;SDS-PAGE showed that the protein is about 17.6 kDa, MALDI-TOF mass spectrometry showed the exact molecular mass was 15.379 kDa. The lectin was found to agglutinate native and trypsinized rabbit erythrocytes. The hemagglutination activity was inhibited by heparin, PSM and albumin. The hemagglutination activity was stable below 50°C and was depend on the presence of divalent cations Ca2+. The crude extract protein could act stimulation effect on rabbit-isolated ileum, and was dose-dependent. When the protein was treated with trypsin or pepsin, respectively, the products could also stimulate ileum.There are huge differences between this purified lectin and the known Heltuba from Helianthus tuberosus L.. The crude extract protein could act stimulation effect on rabbit-isolated ileum no matter treated with or without trypsin or pepsin.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/112842
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Xiong CN,Zhao XM,Yin H,et al. Characterization of lectin from Helianthus tuberosus L. and effects of crude extract proteins on rabbit-isolated ileum[C]. 见:2007 Annual Meeting of the Society for Glycobiology. 美国. 2007-11-11.
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