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学科主题物理化学
Characterization of lectin from Helianthus tuberosus L. and effects of crude extract proteins on rabbit-isolated ileum
Xiong CN(熊川男); Zhao XM(赵小明); Yin H(尹恒); Du YG(杜昱光)
会议名称2007 Annual Meeting of the Society for Glycobiology
会议日期2007-11-11
2007-11-11
会议地点美国
页码116/1
部门归属十八室
主办者glycobiology
英文摘要A lectin from Helianthus tuberosus L. was purified and its effects of crude extract proteins on rabbit-isolated ileum was investigated.DEAD-Sephacel and Sephadex G-200 were used to purified the lectin; SDS-PAGE and MALDI-TOF were used to obtain the molecular mass of the lectin;SDS-PAGE showed that the protein is about 17.6 kDa, MALDI-TOF mass spectrometry showed the exact molecular mass was 15.379 kDa. The lectin was found to agglutinate native and trypsinized rabbit erythrocytes. The hemagglutination activity was inhibited by heparin, PSM and albumin. The hemagglutination activity was stable below 50°C and was depend on the presence of divalent cations Ca2+. The crude extract protein could act stimulation effect on rabbit-isolated ileum, and was dose-dependent. When the protein was treated with trypsin or pepsin, respectively, the products could also stimulate ileum.There are huge differences between this purified lectin and the known Heltuba from Helianthus tuberosus L.. The crude extract protein could act stimulation effect on rabbit-isolated ileum no matter treated with or without trypsin or pepsin.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/112842
专题中国科学院大连化学物理研究所
通讯作者Du YG(杜昱光)
推荐引用方式
GB/T 7714
Xiong CN,Zhao XM,Yin H,et al. Characterization of lectin from Helianthus tuberosus L. and effects of crude extract proteins on rabbit-isolated ileum[C],2007:116/1.
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