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学科主题: 物理化学
题名: Identification of one gene in tobacco leaves induced by oligochitosan
作者: Zhang FY(张付云) ;  Wang QK(汪秋宽) ;  Li W(李伟) ;  Bai XF(白雪芳) ;  Du YG(杜昱光) ;  Zhang YK(张玉奎)
会议名称: 2007 Annual Meeting of the Society for Glycobiology
会议日期: 2007-11-11
出版日期: 2007-11-11
会议地点: 美国
通讯作者: 张付云
部门归属: 十八室
主办者: glycobiology
摘要: Oligochitosan has a variety of biological activities. The presence of oligochitosan triggers a wide range of cellular responses including changes in gene expression and synthesis of PR proteins. To investigate the ability of oligochitosan to affect gene transcription, the messenger RNA (mRNA) differential display technique was applied to the identification and isolation of genes whose transcription was altered in cultured Nicotiana tabacum (var. Samsun NN) plants that were treated with oligochitosan. One gene whose mRNA levels significantly changed in response to oligochitosan was isolated and identified. Reverse Northern blot and RT-PCR analysis indicated that the expression of the gene was up-regulated by oligochitosan. Sequence and homology analysis show that the fragment share 62% identity with polyubiquitin of maize. Because the ubiquitin protein is relevant to plant defense response and hypersensitive response, so these results suggested that oligochitosan can induce plant resistance and its mechanism may be relevant to ubiquitination. These findings also demonstrate the usefulness of mRNA differential display technique for the detection of plant metabolic pathways affected by oligochitosan.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/112844
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhang FY,Wang QK,Li W,et al. Identification of one gene in tobacco leaves induced by oligochitosan[C]. 见:2007 Annual Meeting of the Society for Glycobiology. 美国. 2007-11-11.
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