DICP OpenIR
学科主题物理化学
Identification of one gene in tobacco leaves induced by oligochitosan
Zhang FY(张付云); Wang QK(汪秋宽); Li W(李伟); Bai XF(白雪芳); Du YG(杜昱光); Zhang YK(张玉奎)
会议名称2007 Annual Meeting of the Society for Glycobiology
会议日期2007-11-11
2007-11-11
会议地点美国
页码276/1
部门归属十八室
主办者glycobiology
英文摘要Oligochitosan has a variety of biological activities. The presence of oligochitosan triggers a wide range of cellular responses including changes in gene expression and synthesis of PR proteins. To investigate the ability of oligochitosan to affect gene transcription, the messenger RNA (mRNA) differential display technique was applied to the identification and isolation of genes whose transcription was altered in cultured Nicotiana tabacum (var. Samsun NN) plants that were treated with oligochitosan. One gene whose mRNA levels significantly changed in response to oligochitosan was isolated and identified. Reverse Northern blot and RT-PCR analysis indicated that the expression of the gene was up-regulated by oligochitosan. Sequence and homology analysis show that the fragment share 62% identity with polyubiquitin of maize. Because the ubiquitin protein is relevant to plant defense response and hypersensitive response, so these results suggested that oligochitosan can induce plant resistance and its mechanism may be relevant to ubiquitination. These findings also demonstrate the usefulness of mRNA differential display technique for the detection of plant metabolic pathways affected by oligochitosan.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/112844
专题中国科学院大连化学物理研究所
通讯作者Zhang FY(张付云)
推荐引用方式
GB/T 7714
Zhang FY,Wang QK,Li W,et al. Identification of one gene in tobacco leaves induced by oligochitosan[C],2007:276/1.
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