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学科主题: 物理化学
题名: Marine sponge Hymeniacidon perlevis possesses high diversity Marine sponge Hymeniacidon perlevis possesses high diversity
作者: Xin YJ(信艳娟) ;  Zhang W(张卫)
会议名称: 13th International Biotechnology Symposium and Exhibition
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 张卫
部门归属: 十八室
主办者: 中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
摘要: A total of 59 actinobacteria associated with an intertidal marine sponge Hymeniacidon perlevis collected from the Yellow Sea, China were isolated using eight different media. Species diversity and natural product diversity of isolates were analyzed. Twenty-seven isolates were selected on the basis of their morphology on different media, and assigned to the phylum Actinobacteria by a combination of 16S rRNA gene based restriction enzymes digestion and 16S rRNA gene sequence analysis (Zhang et al., 2008). The 16S rRNA genes of 27 isolates were digested by restriction enzymes Hha I and assigned to different groups according to their restriction enzyme pattern. The phylogenetic analysis based on 16S rRNA gene sequencing showed that the isolates belong to the genera Streptomyces, Nocardiopsis, Micromonospora, Cellulosimicrobium, Gordonia, Nocardia, Prauseria, Pseudonocardia, Saccharomonospora and Microbacterium. Previous studies have isolated strains of the genera Actinoalloteichus, Micromonospora, Nocardiopsis, Nocardia, Rhodococcus, Pseudonocardia, and Streptomyces from the same marine sponge (Zhang et al., 2006). Together, 12 culturable actinobacteria genera have been isolated and identified from the marine sponge H. perlevis. To our knowledge, this is the first report to recover such a high diversity of culturable actinobacteria from any marine sponges. In addition, this is also the first report of actinobacteria classified as Prauseria sp. and Saccharomonospora azurea that have been isolated from amarine sponge. All of the 27 isolates were screened for genes encoding polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS). PKS-I and PKS-II sequences were detected in more than half of the isolates and the different “PKS-I–PKS-II–NRPS” combinations in different isolates belonging to the same species are indicators of their potential natural product diversity and divergent genetic evolution (Pathom-Aree et al., 2006).
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113048
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Xin YJ,Zhang W. Marine sponge Hymeniacidon perlevis possesses high diversity Marine sponge Hymeniacidon perlevis possesses high diversity[C]. 见:13th International Biotechnology Symposium and Exhibition. 中国. 2008-10-12.
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