DICP OpenIR
学科主题物理化学
Isolation and characterization of a lipopeptide biosurfactant from a Pseuomonas sp. associated with the marine sponge Phakeliia sp.
Hong-manHou; Li-liZhai; PengXu; Yu XJ(虞星炬); Zhang W(张卫)
会议名称13th International Biotechnology Symposium and Exhibition
会议日期2008-10-12
2008-10-12
会议地点中国
页码S597/2
部门归属十八室
主办者中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
英文摘要Lipopeptide is a kind of biosurfactant synthesized by microorganisms. In addition to the classical application as surfactant,some unique bioactive functions were also being focused. Marine sponges are well-known as conglomerates of various organisms. Good oil emulsification effect was found during the enrichment culture of the sponge Phakeliia sp. extracts. In this study, bacteria producing biosurfactant were isolated from the sponge by oil spreading technique, and the lipopeptide biosurfactant was extracted and characterized on the surface activity, physicochemical property and bioactivities (Noha et al., 2004). 62 strains isolated from the sponge extracts were proved that three of them had the abilities to produce biosurfactant. The three isolated strain were designated as XP1, XP2, H10. 16S ribosomal DNA sequencing revealed that strains XP1, XP2, H10 belonged to the genus Pseudomonas, Bacillus pumilus and Bacillus subtilis, respectively. Strain XP1 was selected for its best surface activity. Its biosurfactant was identified as a type of lipopeptide and purified by Sephadex LH-20 and ODS column, named as XP-BS. The XP-BS lowered the surface tension of water to 26.5 dyn/cm, and the CMC was 40 M. The XP-BS was stable at pH 2.0–8.5, which was different from other biosurfactants (Christian et al., 2003; Stanislav et al., 2003). Moreover, it showed great thermal stability at 100 ◦C and 121 after incubation for 2 h and 20 min, respectively. The function of XP-BS was not affected at a salt concentration of 1000mM for NaCl and 10mM for CaCl2. XP-BS was tested against a set of standard Gram-positive, Gram-negative, and eukaryotic microorganisms. It exhibited a considerable antimicrobial activity against Staphylococcus aureus and Escherichia coli, a lower activity against epiphyte. XP-BS also showed certain superiority to other chemically synthesized surfactants. All the above results suggested that the applications of XP-BS could be developed commercially.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113052
专题中国科学院大连化学物理研究所
通讯作者Zhang W(张卫)
推荐引用方式
GB/T 7714
Hong-manHou,Li-liZhai,PengXu,et al. Isolation and characterization of a lipopeptide biosurfactant from a Pseuomonas sp. associated with the marine sponge Phakeliia sp.[C],2008:S597/2.
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