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学科主题: 物理化学
题名: Nitrogen limitation on growth, starch accumulation and hydrogen metabolism of marine green alga Platymonas subcordiformis cordiformis
作者: Ji CF(纪超凡) ;  JackLegrand ;  Chen ZA(陈兆安) ;  Zhang W(张卫)
会议名称: 13th International Biotechnology Symposium and Exhibition
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 张卫
部门归属: 十八室
主办者: 中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
摘要: Platymonas subcordiformis, a marine green alga, was demonstrated to photo-biologicallyproducehydrogenwhenregulated by aproton uncoupler CCCP (Carbonyl Cyanide m-Chlorophenylhydrazone).This process composes of two stages: algal cultivation and hydrogen production. In the first stage the green alga growto accumulate biomass and starch. In the second stage, the algal biomass is subjected to a period of dark anaerobic induction of hydrogenase activity and then exposed to light irradiation for photo-evolution of hydrogen using CCCP regulation. CCCP acts to create an anaerobic environment, essential for hydrogen production by reducing the photosystem II (PSII) activity (oxygen evolution) of P. subcordiformis cells below the respiration activity (oxygen consumption of intracellular starch). Therefore the starch content in algal cells determines the final hydrogen yield because hydrogen evolution will effectively stop as soon as it is completely consumed. In this study, it was found that the nitrogen concentration in the medium influenced both the cell growth and also the starch content of P. subcordiformis cells in the first cultivation stage. Nitrogen limitation reduced biomass yield, but increased the starch content, resulting in higher hydrogen yield. During hydrogen production, starch was not completely metabolized into hydrogen, but partially oxidized into formic acid and acetic acid.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113058
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Ji CF,JackLegrand,Chen ZA,et al. Nitrogen limitation on growth, starch accumulation and hydrogen metabolism of marine green alga Platymonas subcordiformis cordiformis[C]. 见:13th International Biotechnology Symposium and Exhibition. 中国. 2008-10-12.
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