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学科主题: 物理化学
题名: Disruption of marine green alga Platymonas subcordiformis for hydrogenase isolation under anaerobic environment
作者: Yan F(彦飞) ;  Chen ZA(陈兆安) ;  Yu XJ(虞星炬) ;  Zhang W(张卫)
会议名称: 13th International Biotechnology Symposium and Exhibition
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 张卫
部门归属: 十八室
主办者: 中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
摘要: A marine unicellular green alga, Platymonas subcordiformis, was found to evolve hydrogen via photolysis of seawater (Guan et al., 2004). To isolate and purify hydrogenase from P. subcordiformis cells, the protocol for algal cell disruption must be operated under anaerobic environment. To maximize the hydrogenase activity recovery, effect of different cell disruption techniques was investigated including ultrasonication, bead-milling, freeze-thawing and chemosmosis methods. Ultrasonication released the highest concentration of total protein at 2.73mg/mL under the condition of 400W output power, 5 s sonication time, and 10 s interval for 20 times. The yield of hydrogenase (1.44 U/g fresh cell and 1.27 U/g fresh cell, respectively) is higher when the cells were disrupted by glass beads or 0.2 % (v/v) Triton X-100. The protein preparation is however more pure by Triton X-100 method, which was used for further purification of hydrogenase (Yan et al., 2008).
语种: 中文
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内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113070
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Yan F,Chen ZA,Yu XJ,et al. Disruption of marine green alga Platymonas subcordiformis for hydrogenase isolation under anaerobic environment[C]. 见:13th International Biotechnology Symposium and Exhibition. 中国. 2008-10-12.
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