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学科主题: 物理化学
题名: Preliminary purification of an iron hydrogenase from marine green alga Platymonas subcordiformis
作者: Yan F(彦飞) ;  Chen ZA(陈兆安) ;  Yu XJ(虞星炬) ;  Zhang W(张卫)
会议名称: 13th International Biotechnology Symposium and Exhibition
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 张卫
部门归属: 十八室
主办者: 中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
摘要: Green algae are the only eukaryotic organisms for which hydrogenase activity has been well documented (Ueno et al., 1999). In this study, hydrogenase from a marine green alga Platymonas subcordiformis (Guan et al., 2004), was purified through three steps under strict anaerobic conditions: ammonium sulfate precipitation, DEAE-52, and Sephadex G-100. Further investigations demonstrated that this partially purified hydrogenase showed a highest in vitro activity of 148 nmol H2 h−1 g Chl−1, at the optimum temperature of 45 ◦C and pH value of 7.5, using reduced methyl viologen as an electron donor. The specific activity of crude extractwas 0.14 U/mg protein, after disrupting algal cells with glass beads at 4 ◦C (Yan et al., 2008). A yield of 85% as judged by the activity was obtained by 50–80% ammonium sulfate precipitation. After ion-exchange chromatography and gel filtration chromatography, two bands with molecular masses of 43 and 32 kDa were revealed on SDS-PAGE, and the former was proved to be hydrogenase. Further study on the amino acid sequences of the hydrogenase is currently under way.
语种: 中文
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内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113072
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Yan F,Chen ZA,Yu XJ,et al. Preliminary purification of an iron hydrogenase from marine green alga Platymonas subcordiformis[C]. 见:13th International Biotechnology Symposium and Exhibition. 中国. 2008-10-12.
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