Subject Area物理化学
Molecular cloning of 14-3-3 genes in marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria
Fu WT(傅晚涛); HongwenLiu; Zhang W(张卫)
Conference Name13th International Biotechnology Symposium and Exhibition
Conference Date2008-10-12
Conference Place中国
Funding Organization中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
AbstractSponges (phylum Porifera) are the phylogenetically oldestmetazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to the microorganisms with no filtering selectivity and about 96% retention rate of bacteria. Understanding the sponge-bacteria interaction is hence of both ecological and biological significance. This study investigated the specific interaction between sponges Hymeniacidon perleve and non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 gene expression of the host sponge. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Reverse-transcript Real-time PCR, the 14-3- 3 gene expression of H. perleve was examined when exposed to three common bacteria in aquatic water—E. coli and two Vibrio spp for different time and dosages. Marine sponge H. perleve could effi- ciently remove E. coli fromthewater columnwithout self-infection; however, Vibrio infected H. perleve at higher dosages. When H. perleve was exposed to E. coli (1.1×107 cells ml−1), V. anguillarum II (1.2×106 cells ml−1) and V. alginolyticus (3.6×105 cells ml−1) for 6 h, the 14-3-3 gene expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67-fold and 2.36-fold. The 14-3-3 gene expression in the E. coli group was not signifi- cantly different from that of the control group. However, no clear trend was observed on the 14-3-3 transcript levels of H. perleve in response to different doses ofV. anguillarum II for different time. The results from this study demonstrated that infectious bacteria can be discriminated by 14-3-3 gene expression of sponge H. perleve. Keywords: Sponge; Hymeniacidon perleve; 14-3-3 mRNA; RT-PCR; Infectious bacteria
WOS IDWOS:000208979402268
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Document Type会议论文
Corresponding AuthorZhang W(张卫)
Recommended Citation
GB/T 7714
Fu WT,HongwenLiu,Zhang W. Molecular cloning of 14-3-3 genes in marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria[C],2008:S557/2.
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