中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: Establishing suspension culture of Cephalotaxus fortunei cells for alkaloid production
作者: Xu XH(徐晓晖) ;  Zhang W(张卫) ;  JinguangXu
会议名称: 13th International Biotechnology Symposium and Exhibition
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 张卫
部门归属: 十八室
主办者: 中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
摘要: Cephalotaxus fortunei Hook. f., a plant indigenous to China, produces valuable anti-cancer alkaloids (e.g. harringtonine and homoharringtonine) for the treatment of different types of leukemia. This plant is difficult to propagate and produces trace amount of alkaloids, therefore in vitro plant cell culture has been suggested as an alternative source of production. However the reported cell cultures of C. fortunei established by far grew very slow, with limited scope for further manipulation of alkaloid biosynthesis. To develop a faster growing cell line, this study focuses on optimizing the growth conditions of a suspension-cultured cell line of C. fortunei Hook. f. that was developed in our earlier studies. The growth conditions tested include the type of medium, culture temperature, light, medium pH, hormone combinations, carbon sources, nitrogen sources and inoculum rate. Based on the growth kinetics and biomass yield, an optimal growth conditionwas established to support a maximum growth index (the ratio of cell concentration on any given day to that on day 0) of 4.24 and 3.96 for both fresh and dry cell weight, respectively on day 21. The attained growth in this study is among the highest when compared to the earlier published data, which provide a good culture system for further studies of bioactive alkaloid biosynthesis.
语种: 中文
Citation statistics: 
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113098
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Xu XH,Zhang W,JinguangXu. Establishing suspension culture of Cephalotaxus fortunei cells for alkaloid production[C]. 见:13th International Biotechnology Symposium and Exhibition. 中国. 2008-10-12.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [徐晓晖]'s Articles
 [张卫]'s Articles
 [JinguangXu]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [徐晓晖]‘s Articles
 [张卫]‘s Articles
 [JinguangXu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace