DICP OpenIR
学科主题物理化学
Establishing suspension culture of Cephalotaxus fortunei cells for alkaloid production
Xu XH(徐晓晖); Zhang W(张卫); JinguangXu
会议名称13th International Biotechnology Symposium and Exhibition
会议日期2008-10-12
2008-10-12
会议地点中国
页码S156/2
部门归属十八室
主办者中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
英文摘要Cephalotaxus fortunei Hook. f., a plant indigenous to China, produces valuable anti-cancer alkaloids (e.g. harringtonine and homoharringtonine) for the treatment of different types of leukemia. This plant is difficult to propagate and produces trace amount of alkaloids, therefore in vitro plant cell culture has been suggested as an alternative source of production. However the reported cell cultures of C. fortunei established by far grew very slow, with limited scope for further manipulation of alkaloid biosynthesis. To develop a faster growing cell line, this study focuses on optimizing the growth conditions of a suspension-cultured cell line of C. fortunei Hook. f. that was developed in our earlier studies. The growth conditions tested include the type of medium, culture temperature, light, medium pH, hormone combinations, carbon sources, nitrogen sources and inoculum rate. Based on the growth kinetics and biomass yield, an optimal growth conditionwas established to support a maximum growth index (the ratio of cell concentration on any given day to that on day 0) of 4.24 and 3.96 for both fresh and dry cell weight, respectively on day 21. The attained growth in this study is among the highest when compared to the earlier published data, which provide a good culture system for further studies of bioactive alkaloid biosynthesis.
语种中文
WOS记录号WOS:000208979400353
引用统计
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113098
专题中国科学院大连化学物理研究所
通讯作者Zhang W(张卫)
推荐引用方式
GB/T 7714
Xu XH,Zhang W,JinguangXu. Establishing suspension culture of Cephalotaxus fortunei cells for alkaloid production[C],2008:S156/2.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[徐晓晖]的文章
[张卫]的文章
[JinguangXu]的文章
百度学术
百度学术中相似的文章
[徐晓晖]的文章
[张卫]的文章
[JinguangXu]的文章
必应学术
必应学术中相似的文章
[徐晓晖]的文章
[张卫]的文章
[JinguangXu]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。