Subject Area物理化学
Establishing suspension culture of Cephalotaxus fortunei cells for alkaloid production
Xu XH(徐晓晖); Zhang W(张卫); JinguangXu
Conference Name13th International Biotechnology Symposium and Exhibition
Conference Date2008-10-12
Conference Place中国
Funding Organization中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
AbstractCephalotaxus fortunei Hook. f., a plant indigenous to China, produces valuable anti-cancer alkaloids (e.g. harringtonine and homoharringtonine) for the treatment of different types of leukemia. This plant is difficult to propagate and produces trace amount of alkaloids, therefore in vitro plant cell culture has been suggested as an alternative source of production. However the reported cell cultures of C. fortunei established by far grew very slow, with limited scope for further manipulation of alkaloid biosynthesis. To develop a faster growing cell line, this study focuses on optimizing the growth conditions of a suspension-cultured cell line of C. fortunei Hook. f. that was developed in our earlier studies. The growth conditions tested include the type of medium, culture temperature, light, medium pH, hormone combinations, carbon sources, nitrogen sources and inoculum rate. Based on the growth kinetics and biomass yield, an optimal growth conditionwas established to support a maximum growth index (the ratio of cell concentration on any given day to that on day 0) of 4.24 and 3.96 for both fresh and dry cell weight, respectively on day 21. The attained growth in this study is among the highest when compared to the earlier published data, which provide a good culture system for further studies of bioactive alkaloid biosynthesis.
WOS IDWOS:000208979400353
Citation statistics
Document Type会议论文
Corresponding AuthorZhang W(张卫)
Recommended Citation
GB/T 7714
Xu XH,Zhang W,JinguangXu. Establishing suspension culture of Cephalotaxus fortunei cells for alkaloid production[C],2008:S156/2.
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