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学科主题: 物理化学
题名: Expression profiling of key genes involved in taxuyunnanine C biosynthesis in suspension cultures of Taxus chinensis by sucrose feeding and double elicitation with 2,3-dihydroxylpropanyl jasmonate
作者: Gao MB(高明波) ;  Zhang W(张卫) ;  Jin MF(金美芳)
会议名称: 13th International Biotechnology Symposium and Exhibition
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 张卫
部门归属: 十八室
主办者: 中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
摘要: Taxus suspension cell culture has the potential to provide a sustainable source of anticancer drug paclitaxel (Taxol®) and other taxoids. In the cell culture of Taxus chinensis, taxuyunnanine C (Tc) is the primary taxoid. To design a rational strategy for redirecting the precursor fluxes from other taxoids into paclitaxel production, this study employed quantitative RT-PCR to understand the dynamic profiling of key biosynthetic pathway genes of palcitaxel and taxoids during the cultureprocess. Six genes (TASY,TDAT, T5 H, T H, TDAT and T10 H) were quantified under a process condition of double elicitation by 2,3-dihydroxylpropanyl jasmonate(DHPJA) (100 M) on days 7 and 12, and sucrose feeding (20 g/L) on day 7. This process treatment led to a high accumulation of taxuyunnanine C (Tc) at 554.46±21.28 mg/L 8 days after the first elicitation, 5.5-fold of that of control. And 9 days after the second elicitation, Tc production was as high as 997.72±1.51 mg/L, 9.7-fold of that of control. The early pathway genes TASY and TDAT were signifi- cantly up-regulated by 182-fold and 98-fold, respectively for the first DHPJA elicitation and by 208-fold and 131-fold, respectively for the second elicitation. The induction after each elicitation lasted for about 24 h before their abundances decreased. Things are some different in the case of the other four genes T5 H, T H, TDAT and T10 H. For gene T H, it was greatly up-regulated by 3061-fold for the first DHPJA elicitation and by 1016-fold for the second elicitation. For the other three genes T5 H, T10 H, T14 H, they were up-regulated by 13-fold, 38-fold and 20-fold, respectively for the firstDHPJA elicitation and by 7-fold, 16-fold and 6-fold, respectively for the second elicitation.
语种: 中文
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内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113102
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Gao MB,Zhang W,Jin MF. Expression profiling of key genes involved in taxuyunnanine C biosynthesis in suspension cultures of Taxus chinensis by sucrose feeding and double elicitation with 2,3-dihydroxylpropanyl jasmonate[C]. 见:13th International Biotechnology Symposium and Exhibition. 中国. 2008-10-12.
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