DICP OpenIR
学科主题物理化学
Experimental studies of the role of different cell types in morphogenesis in marine sponge Hymeniacidon perleve
Sun LM(孙黎明); Zhang W(张卫); Yu XJ(虞星炬); Jin MF(金美芳)
会议名称13th International Biotechnology Symposium and Exhibition
会议日期2008-10-12
2008-10-12
会议地点中国
页码S110/2
部门归属十八室
主办者中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
英文摘要Sponges (Demonspongia, Porifera), the lowest multi-cellular invertebrate animals-sponges are an important model for the research of cell–cell interaction (Imsiecke et al., 1995; Koziol et al., 1998). Archaeocyte is generally considered as the toti/multipotent “stem cells” in sponges. They have the capacity to proliferate and to differentiate into other cell types. To investigate the interaction between archaeocyte with other predominant cell types in marine sponge, such as pinacocyte and collencyte, we separated the archaeocytes, pinacocytes and collencytes from the marine sponge Hymeniacidon perleve by a method composed of differential centrifugation, selective agglomeration, differential adhesion and Ficoll-Vrografin density gradient centrifugation (Sun et al., 2007). The pinacocytes and collencytes (in a ratio of 1:1, denoted together as PCs) mixed with archaeocytes in ratio of 1:2, 1:1 and 2:1, respectivelywere cultured in seawater. The results showed that PCs could aggregate and adhere to the substratum, but they could not develop into small sponge. Archaeocytes could form suspended aggregates, not functional sponges. When PCs and archaeocytes were mixed together, especially in a ratio of 2:1, almost all of the sponge cells aggregated and adhered quickly. Moreover, a series of self-organization and differentiation phenomena could be observed, such as secretion of collagen fibre and silicon spicules, construction of canal systems, outward movement of archaeocytes and final formation of functional sponges. It is suggested that the PCs may play an important role in regulation the biological behaviour, especially differentiation of the archaeocytes and even the morphogenesis of sponge larva.
语种中文
WOS记录号WOS:000208979400255
引用统计
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113106
专题中国科学院大连化学物理研究所
通讯作者Sun LM(孙黎明)
推荐引用方式
GB/T 7714
Sun LM,Zhang W,Yu XJ,et al. Experimental studies of the role of different cell types in morphogenesis in marine sponge Hymeniacidon perleve[C],2008:S110/2.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[孙黎明]的文章
[张卫]的文章
[虞星炬]的文章
百度学术
百度学术中相似的文章
[孙黎明]的文章
[张卫]的文章
[虞星炬]的文章
必应学术
必应学术中相似的文章
[孙黎明]的文章
[张卫]的文章
[虞星炬]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。