Subject Area物理化学
Study on the retention equation in hydrophilic interaction liquid chromatography
Jin GW(金高娃); Xue XY(薛兴亚); Liang XM(梁鑫淼)
Conference NameThe 33rd International Symposium on High Performance Liquid Phase Separations and Related Techniques
Conference Date2008-12-2
Conference Place日本
Alternative Title亲水色谱保留方程研究
Funding Organization日本色谱科学协会
AbstractHydrophilic interaction liquid chromatographic (HILIC) is an effective technique for separating polar compounds. But its retention equation has not been studied systematically yet. In this study, an appropriate retention equation was established by using eight nucleosides as model analytes and by comparing four retention models on six different HILIC columns. Here, three columns were commercial, they were Venusil HILIC (4.6×250 mm, 5 µm, USA), Atlantis HILIC Silica (4.6×250 mm, 5 µm, Waters) and BEH HILIC column (2.1×100 mm, 1.7 µm, Waters); others were homemade with ‘click chemistry’, they were Single-hydroxyl (4.6×150 mm, 5 µm), Multi-hydroxyl (4.6×150 mm, 5 µm) and β-cyclodextrin column (4.6×150 mm, 5 µm). The eight nucleosides were cytidine, adenosine, guanosine, cytosine, uraci, uridine, thymine and adenine. Four retention models were logk=logkw-S*CB, logk=logkB-As/nB*logCB, lnk=a+b*lnCB+c*CB and lnk=c0+c1*CB+C2*CB*CB. As a result, retention equation—lnk=a+b*lnCB+c*CB could be quantitatively described the retention factors with good accuracy in HILIC mode. Based on this equation, the retention times of eight nucleosides under the conditions by other mobile phase can be predicted on each column. All of the predicted relative errors of retention times were smaller than 5%. The established retention model was also successfully applied to predict retention times of the real Traditional Chinese Medicine—Carthaus Tinctorius L. sample. Retention equations of 11 compounds in Carthaus Tinctorius L. were established. The retention times of 11 compounds under other mobile phase conditions were predicted on β-cyclodextrin column and the largest relative error was only 2.96%.
Document Type会议论文
Corresponding AuthorLiang XM(梁鑫淼)
Recommended Citation
GB/T 7714
Jin GW,Xue XY,Liang XM. Study on the retention equation in hydrophilic interaction liquid chromatography[C],2008:261/1.
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