Subject Area物理化学
High-throughput method for preparation and characterization of screening multi-components of TCMs
Liang XM(梁鑫淼); Liu YF(刘艳芳); Zhang XL(张秀莉); Xue XY(薛兴亚); Xu Q(徐青)
Conference Name4th Shanghai International Symposium on Analytical Chemistry
Conference Date2008-9-23
Conference Place中国
Alternative Title中药筛选组分的高通量制备和表征方法
Funding Organization中国化学会和德国慕尼黑国际博览集团
AbstractTraditional Chinese medicines (TCMs) have been attracted widely attention worldwide for its low toxicity and good therapeutical performance. However, it is difficult to explain the curative mechanism of TCMs due to the complexity of TCMs. A high-throughput method has been developed in order to accelerate the study of TCMs. The whole of TCMs were separated into a serious of small fractions containing several compounds by high-throughput preparative chromatography. Then these small fractions, what is called screening multi-components (SMCs), were screened by high-throughput screening (HTS). After biological screening, active multi-components were obtained and could be studied deeply further. This method will accelerate the process of explanation of the curative mechanism of TCMs, which simplifies the complex TCMs by concentrating on those interesting compounds from hundreds of thousands of compounds in TCMs. Standard libraries of SMCs are constructed, which will not only satisfy the need of TCMs, but also will speed up drug discovery by supplying persistent source for HTS. High-throughput preparation of SMCs consists of two steps. Firstly, high-throughput parallel four-channel preparative high-performance liquid chromatography was applied to obtain small fractions. Secondly, high-throughput concentration by trapping the fraction onto a solid phase extraction (SPE) cartridge was developed, which could deal with 48 fractions simultaneously. High-throughput characterization of SMCs can present these SMCs with adequate information that allows for rapid searching and location of components of interest. A high-throughput method with high power in separation has been developed on the application of UPLC/Q-TOF to characterize SMCs, with a run time of 10 min per sample.
Document Type会议论文
Corresponding AuthorLiang XM(梁鑫淼)
Recommended Citation
GB/T 7714
Liang XM,Liu YF,Zhang XL,et al. High-throughput method for preparation and characterization of screening multi-components of TCMs[C],2008:7/1.
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