中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: High-throughput method for preparation and characterization of screening multi-components of TCMs
作者: Liang XM(梁鑫淼) ;  Liu YF(刘艳芳) ;  Zhang XL(张秀莉) ;  Xue XY(薛兴亚) ;  Xu Q(徐青)
会议名称: 4th Shanghai International Symposium on Analytical Chemistry
会议日期: 2008-9-23
出版日期: 2008-09-23
会议地点: 中国
其他题名: 中药筛选组分的高通量制备和表征方法
通讯作者: 梁鑫淼
部门归属: 十八室
主办者: 中国化学会和德国慕尼黑国际博览集团
摘要: Traditional Chinese medicines (TCMs) have been attracted widely attention worldwide for its low toxicity and good therapeutical performance. However, it is difficult to explain the curative mechanism of TCMs due to the complexity of TCMs. A high-throughput method has been developed in order to accelerate the study of TCMs. The whole of TCMs were separated into a serious of small fractions containing several compounds by high-throughput preparative chromatography. Then these small fractions, what is called screening multi-components (SMCs), were screened by high-throughput screening (HTS). After biological screening, active multi-components were obtained and could be studied deeply further. This method will accelerate the process of explanation of the curative mechanism of TCMs, which simplifies the complex TCMs by concentrating on those interesting compounds from hundreds of thousands of compounds in TCMs. Standard libraries of SMCs are constructed, which will not only satisfy the need of TCMs, but also will speed up drug discovery by supplying persistent source for HTS. High-throughput preparation of SMCs consists of two steps. Firstly, high-throughput parallel four-channel preparative high-performance liquid chromatography was applied to obtain small fractions. Secondly, high-throughput concentration by trapping the fraction onto a solid phase extraction (SPE) cartridge was developed, which could deal with 48 fractions simultaneously. High-throughput characterization of SMCs can present these SMCs with adequate information that allows for rapid searching and location of components of interest. A high-throughput method with high power in separation has been developed on the application of UPLC/Q-TOF to characterize SMCs, with a run time of 10 min per sample.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113144
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Liang XM,Liu YF,Zhang XL,et al. High-throughput method for preparation and characterization of screening multi-components of TCMs[C]. 见:4th Shanghai International Symposium on Analytical Chemistry. 中国. 2008-9-23.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [梁鑫淼]'s Articles
 [刘艳芳]'s Articles
 [张秀莉]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [梁鑫淼]‘s Articles
 [刘艳芳]‘s Articles
 [张秀莉]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace