中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: Purification of chitooligosaccharides by using purification factory
作者: Guo ZM(郭志谋) ;  Xiao YS(肖远胜) ;  Liu YF(刘艳芳) ;  Liang T(梁图) ;  Liang XM(梁鑫淼)
会议名称: 13th International Biotechnology Symposium & Exhibition
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
其他题名: 纯化分离工厂制备壳寡糖
通讯作者: 梁鑫淼
部门归属: 十八室
主办者: 中国科学院、中国工程院、教育部、科技部和国家发改委
摘要: Oligosaccharides are being increasingly identified with the biological activities. Chitooligosaccharides (COS), a type of specifically alkali oligosaccharides prepared by chemical and enzymatic hydrolysis of chitosan, was found to have antimicrobial, antibacterial, antitumour and antiangiogenesis properties [1]. But the biological screening is usually performed by using mixture of COS with different degree of polymerization [2], which is an unfavorable way to discover exactly active compounds. Separation and purification of COS is quite difficult due to the highly polarity. A new technique for purification of COS with high efficiency was developed by using purification factory, which is a 4-channel parallel preparative high performance liquid chromatography (HPLC) system. Hydrophilic interaction chromatography (HILIC), an effective HPLC mode for separation of very polar compounds including oligosaccharides [3], was employed to obtain enough retention and resolution of COS under optimized conditions. Four fractions were collected by using mass spectrometry (MS) as collection trigger. The four fractions are four kinds of COS with different degree of polymerization, which is 2, 3, 4, 5, respectively. This new purification technique, together with the purified products, will be very helpful to the research on oligosaccharides. References: [1] Chung, Y. C., Kuo, C. L. and Chen, C. C. 2005. Preparation and important functional properties of water-soluble chitosan produced through Maillard reaction. Bioresource Technology, 13, 1473-1482. [2] Dou, J. L., Tan, C. Y., Du, Y. G., Bai, X. F., Wang, K. Y. and Ma, X. J. 2007. Effects of chitooligosaccharides on rabbit neutrophils in vitro. Carbohydrate Polymers, 69, 209-213. [3] Hemstrom, P. and Irgum, K. 2006. Hydrophilic interaction chromatography. Journal of Separation Science, 29, 1784-1821.
语种: 中文
Citation statistics: 
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113154
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Guo ZM,Xiao YS,Liu YF,et al. Purification of chitooligosaccharides by using purification factory[C]. 见:13th International Biotechnology Symposium & Exhibition. 中国. 2008-10-12.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [郭志谋]'s Articles
 [肖远胜]'s Articles
 [刘艳芳]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [郭志谋]‘s Articles
 [肖远胜]‘s Articles
 [刘艳芳]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace