DICP OpenIR
学科主题物理化学
Purification of chitooligosaccharides by using purification factory
Guo ZM(郭志谋); Xiao YS(肖远胜); Liu YF(刘艳芳); Liang T(梁图); Liang XM(梁鑫淼)
会议名称13th International Biotechnology Symposium & Exhibition
会议日期2008-10-12
2008-10-12
会议地点中国
其他题名纯化分离工厂制备壳寡糖
页码S83/2
部门归属十八室
主办者中国科学院、中国工程院、教育部、科技部和国家发改委
英文摘要Oligosaccharides are being increasingly identified with the biological activities. Chitooligosaccharides (COS), a type of specifically alkali oligosaccharides prepared by chemical and enzymatic hydrolysis of chitosan, was found to have antimicrobial, antibacterial, antitumour and antiangiogenesis properties [1]. But the biological screening is usually performed by using mixture of COS with different degree of polymerization [2], which is an unfavorable way to discover exactly active compounds. Separation and purification of COS is quite difficult due to the highly polarity. A new technique for purification of COS with high efficiency was developed by using purification factory, which is a 4-channel parallel preparative high performance liquid chromatography (HPLC) system. Hydrophilic interaction chromatography (HILIC), an effective HPLC mode for separation of very polar compounds including oligosaccharides [3], was employed to obtain enough retention and resolution of COS under optimized conditions. Four fractions were collected by using mass spectrometry (MS) as collection trigger. The four fractions are four kinds of COS with different degree of polymerization, which is 2, 3, 4, 5, respectively. This new purification technique, together with the purified products, will be very helpful to the research on oligosaccharides. References: [1] Chung, Y. C., Kuo, C. L. and Chen, C. C. 2005. Preparation and important functional properties of water-soluble chitosan produced through Maillard reaction. Bioresource Technology, 13, 1473-1482. [2] Dou, J. L., Tan, C. Y., Du, Y. G., Bai, X. F., Wang, K. Y. and Ma, X. J. 2007. Effects of chitooligosaccharides on rabbit neutrophils in vitro. Carbohydrate Polymers, 69, 209-213. [3] Hemstrom, P. and Irgum, K. 2006. Hydrophilic interaction chromatography. Journal of Separation Science, 29, 1784-1821.
语种中文
WOS记录号WOS:000208979400193
引用统计
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113154
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼)
推荐引用方式
GB/T 7714
Guo ZM,Xiao YS,Liu YF,et al. Purification of chitooligosaccharides by using purification factory[C],2008:S83/2.
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