DICP OpenIR
Subject Area物理化学
Purification of chitooligosaccharides by using purification factory
Guo ZM(郭志谋); Xiao YS(肖远胜); Liu YF(刘艳芳); Liang T(梁图); Liang XM(梁鑫淼)
Conference Name13th International Biotechnology Symposium & Exhibition
Conference Date2008-10-12
2008-10-12
Conference Place中国
Alternative Title纯化分离工厂制备壳寡糖
PagesS83/2
Department十八室
Funding Organization中国科学院、中国工程院、教育部、科技部和国家发改委
AbstractOligosaccharides are being increasingly identified with the biological activities. Chitooligosaccharides (COS), a type of specifically alkali oligosaccharides prepared by chemical and enzymatic hydrolysis of chitosan, was found to have antimicrobial, antibacterial, antitumour and antiangiogenesis properties [1]. But the biological screening is usually performed by using mixture of COS with different degree of polymerization [2], which is an unfavorable way to discover exactly active compounds. Separation and purification of COS is quite difficult due to the highly polarity. A new technique for purification of COS with high efficiency was developed by using purification factory, which is a 4-channel parallel preparative high performance liquid chromatography (HPLC) system. Hydrophilic interaction chromatography (HILIC), an effective HPLC mode for separation of very polar compounds including oligosaccharides [3], was employed to obtain enough retention and resolution of COS under optimized conditions. Four fractions were collected by using mass spectrometry (MS) as collection trigger. The four fractions are four kinds of COS with different degree of polymerization, which is 2, 3, 4, 5, respectively. This new purification technique, together with the purified products, will be very helpful to the research on oligosaccharides. References: [1] Chung, Y. C., Kuo, C. L. and Chen, C. C. 2005. Preparation and important functional properties of water-soluble chitosan produced through Maillard reaction. Bioresource Technology, 13, 1473-1482. [2] Dou, J. L., Tan, C. Y., Du, Y. G., Bai, X. F., Wang, K. Y. and Ma, X. J. 2007. Effects of chitooligosaccharides on rabbit neutrophils in vitro. Carbohydrate Polymers, 69, 209-213. [3] Hemstrom, P. and Irgum, K. 2006. Hydrophilic interaction chromatography. Journal of Separation Science, 29, 1784-1821.
Language中文
WOS IDWOS:000208979400193
Citation statistics
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/113154
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼)
Recommended Citation
GB/T 7714
Guo ZM,Xiao YS,Liu YF,et al. Purification of chitooligosaccharides by using purification factory[C],2008:S83/2.
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